Minnesota/7 July 2008
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|4. '''PROBLEM:''' Gels under UV light show no PCR DNA bands. Must redo PCR and run through another gel. | |4. '''PROBLEM:''' Gels under UV light show no PCR DNA bands. Must redo PCR and run through another gel. | ||
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- | |5. ''' | + | |5. '''Double Digests''': Digest Terminator, base vector, and promoter. Run through gel to check size. |
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- | | '''a.''' Received DB3.1 competent E. Coli cells. Transformed base vectors into DB3.1 cells. | + | |[[Image:digest.JPG|600px||center|thumb|Digest diagram]] |
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+ | |6. '''Meeting with Yiannis''': 1pm meeting with grad students and advisor. Discussed: | ||
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+ | | '''a.''' Received DB3.1 competent E. Coli cells. Transformed base vectors containing ccdB gene into DB3.1 cells that are resistant to the lethal ccdB gene. | ||
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|'''b.''' Transformed TOP10 cells to the DH5alpha cells because want all DNA to be inside same cells. Problem resolved - DH5alpha cells are fine, but the amount of DNA sent in iGEM notebook was too little. | |'''b.''' Transformed TOP10 cells to the DH5alpha cells because want all DNA to be inside same cells. Problem resolved - DH5alpha cells are fine, but the amount of DNA sent in iGEM notebook was too little. |
Latest revision as of 21:18, 23 July 2008
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