Minnesota/7 July 2008
From 2008.igem.org
(Difference between revisions)
Emartin9808 (Talk | contribs) |
Emartin9808 (Talk | contribs) |
||
(One intermediate revision not shown) | |||
Line 17: | Line 17: | ||
|- | |- | ||
|5. '''Double Digests''': Digest Terminator, base vector, and promoter. Run through gel to check size. | |5. '''Double Digests''': Digest Terminator, base vector, and promoter. Run through gel to check size. | ||
+ | |- | ||
+ | |[[Image:digest.JPG|600px||center|thumb|Digest diagram]] | ||
|- | |- | ||
|6. '''Meeting with Yiannis''': 1pm meeting with grad students and advisor. Discussed: | |6. '''Meeting with Yiannis''': 1pm meeting with grad students and advisor. Discussed: | ||
|- | |- | ||
- | | '''a.''' Received DB3.1 competent E. Coli cells. Transformed base vectors into DB3.1 cells. | + | | '''a.''' Received DB3.1 competent E. Coli cells. Transformed base vectors containing ccdB gene into DB3.1 cells that are resistant to the lethal ccdB gene. |
|- | |- | ||
|'''b.''' Transformed TOP10 cells to the DH5alpha cells because want all DNA to be inside same cells. Problem resolved - DH5alpha cells are fine, but the amount of DNA sent in iGEM notebook was too little. | |'''b.''' Transformed TOP10 cells to the DH5alpha cells because want all DNA to be inside same cells. Problem resolved - DH5alpha cells are fine, but the amount of DNA sent in iGEM notebook was too little. |
Latest revision as of 21:18, 23 July 2008
Back to Notebook Home | |
Go to Previous Day (July 4) | Go to Next Day (July 8) |