"
Team:The University of Alberta/24 July 2008
From 2008.igem.org
(Difference between revisions)
| Line 8: | Line 8: | ||
**Then ligated the B0034+TETR with LacIQERE giving us a complete system 2. | **Then ligated the B0034+TETR with LacIQERE giving us a complete system 2. | ||
**PCRed the digested product i.e tetR+BOO34 | **PCRed the digested product i.e tetR+BOO34 | ||
| - | *Digested pTet with Spe/Pst. | + | *Digested pTet with Spe/Pst. Column purified the digested product. |
*Digested RFP with Xba/Pst. | *Digested RFP with Xba/Pst. | ||
==System 3== | ==System 3== | ||
*Digested 5ul of J61003 with Xba/Pst; this will let us insert the Tryp biobrick digested and ligated yesterday | *Digested 5ul of J61003 with Xba/Pst; this will let us insert the Tryp biobrick digested and ligated yesterday | ||
**Ran the digested J6 on a gel; gel purified | **Ran the digested J6 on a gel; gel purified | ||
Revision as of 21:17, 24 July 2008
General
- Did mini-preps of J61003 because we were running out
- Digested BisDA/B with single digests using AgeI and NgoMIV to test the enzymes because weve been getting cruddy digests when using both of them
System 2
Today we have taken the ligation from yesterday and transformed it as well as digested it.
- if all goes well the digested product should be B0034+TETR
- Column Purified the digested product.
- Then ligated the B0034+TETR with LacIQERE giving us a complete system 2.
- PCRed the digested product i.e tetR+BOO34
- Digested pTet with Spe/Pst. Column purified the digested product.
- Digested RFP with Xba/Pst.
System 3
- Digested 5ul of J61003 with Xba/Pst; this will let us insert the Tryp biobrick digested and ligated yesterday
- Ran the digested J6 on a gel; gel purified
