"
Alberta NINT/22 July 2008
From 2008.igem.org
(Difference between revisions)
(New page: < Back to notebook < Previous entry lab work (SD): Results from 21/07/08 overnight cultures in 96-well plate...) |
|||
| Line 5: | Line 5: | ||
lab work (SD): | lab work (SD): | ||
Results from 21/07/08 overnight cultures in 96-well plate: No growth seen in double-plasmid wells. | Results from 21/07/08 overnight cultures in 96-well plate: No growth seen in double-plasmid wells. | ||
| - | Growth visible in K102022 and XL1-B wells. | + | Growth visible in K102022 and XL1-B wells. Put 96-well plate back at 37 C. |
Test transformation technique by adding 1.0 ul of K102032 and 1.0 ul of K102034 to XL1-B cells and | Test transformation technique by adding 1.0 ul of K102032 and 1.0 ul of K102034 to XL1-B cells and | ||
plating 10 ul on LB+amp50, 10 ul on LB+kan35, and 100ul on LB+amp50+kan35. | plating 10 ul on LB+amp50, 10 ul on LB+kan35, and 100ul on LB+amp50+kan35. | ||
[[Alberta_NINT/23_July_2008 | Next entry >]] | [[Alberta_NINT/23_July_2008 | Next entry >]] | ||
Revision as of 16:23, 25 July 2008
lab work (SD):
Results from 21/07/08 overnight cultures in 96-well plate: No growth seen in double-plasmid wells.
Growth visible in K102022 and XL1-B wells. Put 96-well plate back at 37 C.
Test transformation technique by adding 1.0 ul of K102032 and 1.0 ul of K102034 to XL1-B cells and
plating 10 ul on LB+amp50, 10 ul on LB+kan35, and 100ul on LB+amp50+kan35.
