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Team:The University of Alberta/25 July 2008
From 2008.igem.org
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*Sequenced TetR-RBS. Results yet to come. | *Sequenced TetR-RBS. Results yet to come. | ||
| - | *Digested PCR product of TetR-RBS with Xba/Pst. | + | *Digested PCR product of TetR-RBS with Xba/Pst. Unfortunately, digestions didn't work at all. Redo on Monday. |
*Transformed the ligation of J6-Tryp. | *Transformed the ligation of J6-Tryp. | ||
*Transformed LacIQERE-TetR.RBS. | *Transformed LacIQERE-TetR.RBS. | ||
| - | *tested digesting activity of AgeI and NgoMIV in buffer NEB4 | + | *tested digesting activity of AgeI and NgoMIV in buffer NEB4 using BisDA DNA |
Latest revision as of 21:14, 25 July 2008
- Sequenced TetR-RBS. Results yet to come.
- Digested PCR product of TetR-RBS with Xba/Pst. Unfortunately, digestions didn't work at all. Redo on Monday.
- Transformed the ligation of J6-Tryp.
- Transformed LacIQERE-TetR.RBS.
- tested digesting activity of AgeI and NgoMIV in buffer NEB4 using BisDA DNA
