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Team:The University of Alberta/22 May 2008
From 2008.igem.org
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<u>'''Benny'''</u> | <u>'''Benny'''</u> | ||
<ul> | <ul> | ||
| - | <li>Prepared digestions for B0043 using single digestion method (enzyme PSTI), double digestion method (enzymes PSTI/EcoRI); ran | + | <li>Prepared digestions for B0043 using single digestion method (enzyme PSTI), double digestion method (enzymes PSTI/EcoRI) |
| + | </ul> | ||
| + | |||
| + | <u>'''Saima'''</u><br> | ||
| + | <ul><li>Completed sequencing from last day. | ||
| + | <li> Transformed and plated I0500, GFP, I725025, I725099, I725022 (forgot to add LB, redid below) | ||
| + | <li> Religated I725025, I725099, I725022; retransformed and replated I0500, GFP</ul> | ||
| + | |||
| + | <u>'''Winnie'''</u> | ||
| + | <ul><li>Did mini-preps on I725023 | ||
| + | <li>Transformed I725021 and RFP | ||
| + | <li>Digested I725023 with Xba and Pst, ran on a gel</ul> | ||
| + | |||
| + | ==Lab Tip of the Day== | ||
| + | Remember, when you are transforming E.Coli, always add 900ul of LB media after you heat shock your cells. The competent cells are in a fragile state, and they need the media to repair the damage that has been done to them. If you forget to add the LB, a small fraction of the cells may survive, but you will end up with a pretty crummy transformation efficency and will likely have to repeat the whole thing. | ||
Latest revision as of 22:31, 23 May 2008
Volunteers Scheduled for Today
10-3 BH
5-9 KR
6-9 DL
Things to do:
Finish processing sequence reactions of 021,022,023,025,099
Transform ligation reactions
Transform I0500
Stuff we did Today
Chris
- I got the calander working properly! I just added "title=Team:The_University_of_Alberta" to the code from the calander and now each date links to pages under our own Namespace. No more worrying that other teams might erase our notes!
- Working on an Optimization Tutorial for those of you who want to work on building BioBricks. The website for optimizing sequences for use in E.Coli is kinda tricky so the tutorial should make it a bit easier to use.
Benny
- Prepared digestions for B0043 using single digestion method (enzyme PSTI), double digestion method (enzymes PSTI/EcoRI)
Saima
- Completed sequencing from last day.
- Transformed and plated I0500, GFP, I725025, I725099, I725022 (forgot to add LB, redid below)
- Religated I725025, I725099, I725022; retransformed and replated I0500, GFP
Winnie
- Did mini-preps on I725023
- Transformed I725021 and RFP
- Digested I725023 with Xba and Pst, ran on a gel
Lab Tip of the Day
Remember, when you are transforming E.Coli, always add 900ul of LB media after you heat shock your cells. The competent cells are in a fragile state, and they need the media to repair the damage that has been done to them. If you forget to add the LB, a small fraction of the cells may survive, but you will end up with a pretty crummy transformation efficency and will likely have to repeat the whole thing.
