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Team:The University of Alberta/23 May 2008
From 2008.igem.org
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<li> Finish the Binary Vector | <li> Finish the Binary Vector | ||
</ul> | </ul> | ||
| + | == The legacy of the Purple Russian and the Blue Ox== | ||
| + | The Purple Russian being the purple GFP<br> | ||
| + | The Blue Ox is two enzymes that Mike dropped off that will cause our bacteria to go blue | ||
| + | |||
| + | Today <br> | ||
| + | We recieved the purple russian and blue ox from mike (in pUC57) and will transform them into bacteria to make glycerol stocks. We will then design primers to turn these into biobricks.<br> | ||
| + | |||
| + | ==What we accomplished today== | ||
| + | <u>'''Chris'''</u>: | ||
| + | <ul><li>Jason and I transformed Purple Russian (ASFSP95_CDS) and Blue Ox (Dioxygenase and Tryp.) into XL10. | ||
| + | <li>Plated out the transformants (10ul, 100ul, 400ul per sample) and placed in the incubator. I'll come in tomorrow to take them out and place them in the fridge to make sure they dont end up as a lawn of bacteria when we come in on Monday.</ul> | ||
| + | |||
| + | <u>'''Winnie'''</u> | ||
| + | <ul><li>Digested I725023 with Xba and Pst, ran them on a gel and gel extracted. Spec'd the DNA and had a concentration of only 0.5ug/mL....saved the original miniprep, so will digest again and gex extract on Monday. | ||
| + | <li> Did Colony PCR on 4 colonies containing I725021</ul> | ||
| + | |||
| + | <u>'''Saima'''</u> | ||
| + | <ul><li>Did Colony PCR on I0500 GFP, I725022, I725025 and I725099. Lots of Colony PCR. Also streaked out the colonies on sector plates. | ||
| + | <li>Ran colony PCR out on a gel | ||
| + | <li>Made 500mL of LB Amp plates | ||
| + | <li> Cleaned up the lab</ul> | ||
| + | |||
| + | <u>'''Anthony and Kelly'''</u> | ||
| + | <ul><li>Gel purification of Colony PCR bands and I72053 insert with QIAquick Gel Purification Kit</ul> | ||
| + | |||
| + | ==Lab Tip Of The Day== | ||
| + | When you're running a gel, be sure not to leave it running over night. Remember, nothing is a perfect conductor, and that includes the gel buffer. Some of the electric current is given off as heat. Running your gel for too long can thus cause three problems:<ul> | ||
| + | 1.Your bands will run off the bottom and you will lose them<br> | ||
| + | 2.Your gel will melt and this is a pain to clean up<br> | ||
| + | 3.Your gel/gel aparatus can ignite and the lab can burn down. This is something you may want to avoid.</ul> | ||
Latest revision as of 01:15, 24 May 2008
Contents |
Volunteers Scheduled for Today
5-8 AL
5-8 KR
Today
- Work on the Purple Russian and The Blue Ox
- Finish the Binary Vector
The legacy of the Purple Russian and the Blue Ox
The Purple Russian being the purple GFP
The Blue Ox is two enzymes that Mike dropped off that will cause our bacteria to go blue
Today
We recieved the purple russian and blue ox from mike (in pUC57) and will transform them into bacteria to make glycerol stocks. We will then design primers to turn these into biobricks.
What we accomplished today
Chris:
- Jason and I transformed Purple Russian (ASFSP95_CDS) and Blue Ox (Dioxygenase and Tryp.) into XL10.
- Plated out the transformants (10ul, 100ul, 400ul per sample) and placed in the incubator. I'll come in tomorrow to take them out and place them in the fridge to make sure they dont end up as a lawn of bacteria when we come in on Monday.
Winnie
- Digested I725023 with Xba and Pst, ran them on a gel and gel extracted. Spec'd the DNA and had a concentration of only 0.5ug/mL....saved the original miniprep, so will digest again and gex extract on Monday.
- Did Colony PCR on 4 colonies containing I725021
Saima
- Did Colony PCR on I0500 GFP, I725022, I725025 and I725099. Lots of Colony PCR. Also streaked out the colonies on sector plates.
- Ran colony PCR out on a gel
- Made 500mL of LB Amp plates
- Cleaned up the lab
Anthony and Kelly
- Gel purification of Colony PCR bands and I72053 insert with QIAquick Gel Purification Kit
Lab Tip Of The Day
When you're running a gel, be sure not to leave it running over night. Remember, nothing is a perfect conductor, and that includes the gel buffer. Some of the electric current is given off as heat. Running your gel for too long can thus cause three problems:-
1.Your bands will run off the bottom and you will lose them
2.Your gel will melt and this is a pain to clean up
3.Your gel/gel aparatus can ignite and the lab can burn down. This is something you may want to avoid.
