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Team:ESBS-Strasbourg/24 July 2008
From 2008.igem.org
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(→WetLab) |
(→WetLab) |
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*tetR, lacI PCR on BB (Primers, VFPrimers) | *tetR, lacI PCR on BB (Primers, VFPrimers) | ||
*Pfu Pol PCR on pGEX lacI containing plasmid | *Pfu Pol PCR on pGEX lacI containing plasmid | ||
| + | *ON of BB: | ||
| + | **J63002 | ||
| + | **J63003 | ||
| + | **J63005 | ||
| + | **J63008 | ||
''Sandra'' | ''Sandra'' | ||
* Maxiprep of plasmid p10101A2 with final amount of 70 µg and a good quality (ratio 260/280 = 1,7)<br> | * Maxiprep of plasmid p10101A2 with final amount of 70 µg and a good quality (ratio 260/280 = 1,7)<br> | ||
Latest revision as of 13:59, 28 July 2008
Contents |
DryLab
Modeling
WetLab
Manuel
- tetR, lacI PCR on BB (Primers, VFPrimers)
- Pfu Pol PCR on pGEX lacI containing plasmid
- ON of BB:
- J63002
- J63003
- J63005
- J63008
Sandra
- Maxiprep of plasmid p10101A2 with final amount of 70 µg and a good quality (ratio 260/280 = 1,7)
=> find them in the -20°C fridge in our lab in tubes with different concentrations
Paul
Digestion of tetR/lacI/c1 plasmids, in order to check if the biobricks are really there. Used of EcoR1 and Spe1.
=> no results, probably not enough DNA for the digestion. To redo next day.
General
- Animation (MM)
