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Team:ESBS-Strasbourg/25 July 2008
From 2008.igem.org
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== WetLab == | == WetLab == | ||
| - | ''Paul'' | + | ''Manuel'' |
| + | *PCR VFprimer lacI BB | ||
| + | *Gelpurification | ||
| + | *2nd PCR | ||
| + | ** on product of first PCR with original primer | ||
| + | ** original primer directly on BB plasmid | ||
| + | *Miniprep of J6300X (x=2,3,5,8) | ||
| + | *Verification gel of miniprep | ||
| + | ''Paul'' <br> | ||
-Digestion of tetR, lacI, c1, in order to check if the biobricks are really in the plasmid. Digestion with EcoR1 and Spe1 <br> | -Digestion of tetR, lacI, c1, in order to check if the biobricks are really in the plasmid. Digestion with EcoR1 and Spe1 <br> | ||
Latest revision as of 14:03, 28 July 2008
Contents |
DryLab
Modeling
WetLab
Manuel
- PCR VFprimer lacI BB
- Gelpurification
- 2nd PCR
- on product of first PCR with original primer
- original primer directly on BB plasmid
- Miniprep of J6300X (x=2,3,5,8)
- Verification gel of miniprep
Paul
-Digestion of tetR, lacI, c1, in order to check if the biobricks are really in the plasmid. Digestion with EcoR1 and Spe1
-Digestion of the PCR products tetR, lexA, Gal4, in order to have them in a silver standard and to insert them into the vector p1010 1A2. In this way, this vector is also digested for future insertion of biobricks. Enzymes used: EcoR1 and Spe1.
General
- Rechecked Primers to order (MM)
