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Team:ESBS-Strasbourg/28 July 2008
From 2008.igem.org
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* Hybridization of the oligos for the linker sequence and the MCS | * Hybridization of the oligos for the linker sequence and the MCS | ||
* Blunting of the linker and Phenol-Chloroform-Precipitation | * Blunting of the linker and Phenol-Chloroform-Precipitation | ||
| + | |||
| + | ''Paul'' | ||
| + | -Digestion of lacI/lexA/tetR/gal4/plasmid death, by EcoR1 and Spe1 <br> | ||
| + | -gel migration and purification of those product <br> | ||
| + | -ligation overnight at 21°C <br> | ||
| + | |||
| + | =>Cut with the wrong enzymes; now use of Spe1 and Xba1 | ||
== General == | == General == | ||
Latest revision as of 12:44, 29 July 2008
Contents |
DryLab
Modeling
WetLab
Sandra
- Hybridization of the oligos for the linker sequence and the MCS
- Blunting of the linker and Phenol-Chloroform-Precipitation
Paul
-Digestion of lacI/lexA/tetR/gal4/plasmid death, by EcoR1 and Spe1
-gel migration and purification of those product
-ligation overnight at 21°C
=>Cut with the wrong enzymes; now use of Spe1 and Xba1
General
Sandra
- Slides for presentation
Marius
- Presentation (Layout,Assembly)
