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University of Sheffield/18 July 2008
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| + | {| style="color:yellow;background-color:cyan;" cellpadding="5" cellspacing="2" border="2" bordercolor="blue" width="64%" align="center" | ||
| + | !align="center"|[[Team:University_of_Sheffield |Home]] | ||
| + | !align="center"|[[Team:University_of_Sheffield / Team |The Team]] | ||
| + | !align="center"|[[Team:University_of_Sheffield /Project|The Project]] | ||
| + | !align="center"|[[Team:University_of_Sheffield / Parts submitted to the Registry|Parts submitted to the Registry]] | ||
| + | !align="center"|[[Team:University_of_Sheffield /Modelling| Modelling]] | ||
| + | !align="center"|[[Team:University_of_Sheffield /Notebook| Notebook]] | ||
| + | |} | ||
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| - | | style="text-align: center;" | [[Image:Meeting_Shef.png]] | + | | style="text-align: center;" | [[Image:Meeting_Shef.png | 500px]] |
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| - | ! | + | ! Low cell density (''V.harveyi'') |
|- | |- | ||
| - | | style=" | + | | style="background-color: green;" | <span style="color: white;">It is seen from the cascading pathway (''above'') how the gene A-E is not synthesized as repressor gene attaches with the promoter. The pathway starts from autoinducer receptor. </span> |
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| + | {| border="5" cellspacing="5" cellpadding="2" | ||
| + | | style="text-align: center;" | [[Image:Meeting_Shef1.png | 500px]] | ||
| + | |- | ||
| + | ! Low cell density (''V.Cholera'') | ||
| + | |- | ||
| + | | style="background-color: green;" | <span style="color: white;">The cascading pathway(''above'') is similiar to the one of ''V.harveyi'' but with a slight difference. In ''V.Cholera'' HapR i.e. the promoter site is not intact with the repressor gene thus transcription of virulence takes place. </span> | ||
| + | |} | ||
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| + | |||
| + | ==Conclusion== | ||
| + | As we intend to modify the E-Coli in a way so that its receptor can sense ''V.Cholera & Legionella''. The problem here is both V.Cholera and Legionella have cascading pathways where reactions take place in a series of steps whereas in E.Coli all reactions are simultaneous. So we might have to introduce '''TWO''' plasmids in E.Coli to perform the job. | ||
Latest revision as of 12:49, 29 July 2008
| Home | The Team | The Project | Parts submitted to the Registry | Modelling | Notebook |
|---|
|
| Low cell density (V.harveyi) |
|---|
| It is seen from the cascading pathway (above) how the gene A-E is not synthesized as repressor gene attaches with the promoter. The pathway starts from autoinducer receptor. |
|
| Low cell density (V.Cholera) |
|---|
| The cascading pathway(above) is similiar to the one of V.harveyi but with a slight difference. In V.Cholera HapR i.e. the promoter site is not intact with the repressor gene thus transcription of virulence takes place. |
Conclusion
As we intend to modify the E-Coli in a way so that its receptor can sense V.Cholera & Legionella. The problem here is both V.Cholera and Legionella have cascading pathways where reactions take place in a series of steps whereas in E.Coli all reactions are simultaneous. So we might have to introduce TWO plasmids in E.Coli to perform the job.
