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Team:KULeuven/29 July 2008
From 2008.igem.org
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=== Wet Lab === | === Wet Lab === | ||
| - | Only one ligation succeeded (R0084+B0032). We think that the others failed because the vectors weren't properly cut. Therefore, we | + | * Only one ligation succeeded (R0084+B0032). We think that the others failed because the vectors weren't properly cut. Therefore, we tried another digest. This time we cut the vector with ''Xba'' I, and only when this worked, we cut it with ''Eco''R I. We did this because ''Xba'' I has problems with cleaving close to the ends of DNA fragments. The problem is that ''Xba'' I doesn't seem to cut. Perhaps there's something wrong with the enzyme. We are trying to overcome this problem by allowing the restriction with ''Xba'' I to continue overnight. |
| + | * Some more plasmids were isolated from the cells using the Qiagen MiniPrep Kit. It concerns parts used in the filter and in the inverter (J23022, J23109, J23032, I712074, B0015, R0084, B0032 and B0034, C0012, R0011, F1610). We also digested some of these isolated plasmids. The ones that were cut with ''Eco''R I and ''Spe'' I succeeded (R0084, J23109 and I712074) and were cut out of the agarose gel and purified. The ones that were cut with ''Xba'' I failed (J23022, J23032 and B0015) - we didn't get the expected result with gel electrophoresis. | ||
=== Dry Lab === | === Dry Lab === | ||
Revision as of 18:09, 29 July 2008
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Contents |
Lab Work
Wet Lab
- Only one ligation succeeded (R0084+B0032). We think that the others failed because the vectors weren't properly cut. Therefore, we tried another digest. This time we cut the vector with Xba I, and only when this worked, we cut it with EcoR I. We did this because Xba I has problems with cleaving close to the ends of DNA fragments. The problem is that Xba I doesn't seem to cut. Perhaps there's something wrong with the enzyme. We are trying to overcome this problem by allowing the restriction with Xba I to continue overnight.
- Some more plasmids were isolated from the cells using the Qiagen MiniPrep Kit. It concerns parts used in the filter and in the inverter (J23022, J23109, J23032, I712074, B0015, R0084, B0032 and B0034, C0012, R0011, F1610). We also digested some of these isolated plasmids. The ones that were cut with EcoR I and Spe I succeeded (R0084, J23109 and I712074) and were cut out of the agarose gel and purified. The ones that were cut with Xba I failed (J23022, J23032 and B0015) - we didn't get the expected result with gel electrophoresis.
Dry Lab
Modeling
Mainly chillin' out, memory was finished and complete model is functional.
Wiki
Modeling section got taken care of some more, work on new dropdown menu proceeds...
Remarks
Quote Of The Day
Sigrid: Het is te hopen dat de getransduceerde cellen een groeischeut krijgen. Stefanie: Daar wacht ik nu al 22 jaar op!
