Team:University of Ottawa/19 July 2008

From 2008.igem.org

(Difference between revisions)
(New page: __TOC__ ==Today in the lab== '''Dan''' :'''Ligation''' ::<li>Spiked my overnight ligation with 1 uL ATP and 1 uL enzyme.)
 
(2 intermediate revisions not shown)
Line 1: Line 1:
 +
{|######Navigational Bar#######
 +
|}
 +
<html xmlns="http://www.w3.org/1999/xhtml">
 +
<head>
 +
<meta http-equiv="Content-Type" content="text/html; charset=UTF-8" />
 +
<title>Untitled Document</title>
 +
<script src="http://www.sysbiolab.uottawa.ca/igem/wiki/SpryAssets/SpryMenuBar.js" type="text/javascript"></script>
 +
<link href="http://www.sysbiolab.uottawa.ca/igem/wiki/SpryAssets/SpryMenuBarHorizontal.css" rel="stylesheet" type="text/css" />
 +
</head>
 +
 +
<body>
 +
 +
<ul id="MenuBar1" class="MenuBarHorizontal">
 +
  <li><a class=MenuBarItemSubmenu href="https://2008.igem.org/Team:University_of_Ottawa">Home</a>
 +
      <ul>
 +
        <li><a href="https://2008.igem.org/Team:University_of_Ottawa#Welcome_to_the_uOttawa_Team_Wiki.21">Welcome</a></li>
 +
        <li><a href="https://2008.igem.org/Team:University_of_Ottawa#Announcements">Announcements</a></li>
 +
      </ul>
 +
  <li><a class=MenuBarItemSubmenu href="https://2008.igem.org/Team:University_of_Ottawa/Team">The Team</a>
 +
      <ul>
 +
        <li><a href="https://2008.igem.org/Team:University_of_Ottawa/Team#Who_we_are">Who We Are</a>
 +
            <ul>
 +
              <li><a href="https://2008.igem.org/Team:University_of_Ottawa/Team#Advisors">Advisors</a></li>
 +
              <li><a href="https://2008.igem.org/Team:University_of_Ottawa/Team#Undergrads">Undergrads</a></li>
 +
            </ul>
 +
        </li>
 +
        <li><a href="https://2008.igem.org/Team:University_of_Ottawa/Team#What_we_did">What We've Done</a></li>
 +
        <li><a href="https://2008.igem.org/Team:University_of_Ottawa/Team#Where_we.27re_from">Where We're From</a></li>
 +
        <li><a href="https://2008.igem.org/Team:University_of_Ottawa/Team#Contact_Us">Contact Us</a></li>
 +
       
 +
      </ul>
 +
  </li>
 +
  <li><a class="MenuBarItemSubmenu" href="https://2008.igem.org/Team:University_of_Ottawa/Project">The Project</a>
 +
      <ul>
 +
        <li><a href="https://2008.igem.org/Team:University_of_Ottawa/Project#Project_Overview">Overview</a></li>
 +
        <li><a href="https://2008.igem.org/Team:University_of_Ottawa/Project#Pulsate_Gene_Expression">Expression</a></li>
 +
        <li><a href="https://2008.igem.org/Team:University_of_Ottawa/Project#Cell-to-Cell_Communication">Communication</a></li>
 +
        <li><a href="https://2008.igem.org/Team:University_of_Ottawa/Project#Oscillatory_Dynamics">Oscillation</a></li>
 +
        <li><a href="https://2008.igem.org/Team:University_of_Ottawa/Project#Applications">Application</a></li>
 +
        <li><a href="https://2008.igem.org/Team:University_of_Ottawa/Project#Journal_Club">Journal Club</a></li>
 +
 +
      </ul>
 +
  </li>
 +
  <li><a href="https://2008.igem.org/Team:University_of_Ottawa/Parts">BioBricks</a></li>
 +
  <li><a href="https://2008.igem.org/Team:University_of_Ottawa/Modeling">Modeling</a></li>
 +
  <li><a class="MenuBarItemSubmenu" href="https://2008.igem.org/Team:University_of_Ottawa/Wet_Lab">Wet Lab</a>
 +
    <ul>
 +
        <li><a href="https://2008.igem.org/Team:University_of_Ottawa/Lab_Protocols">Lab Protocols</a></li>
 +
        <li><a href="https://2008.igem.org/Team:University_of_Ottawa/WetWare">WetWare</a></li>
 +
    </ul>
 +
  <li><a href="https://2008.igem.org/Team:University_of_Ottawa/Notebook">Notebook</a></li>
 +
  <li><a class="MenuBarItemSubmenu" href="https://2008.igem.org/Team:University_of_Ottawa/Sponsors">Sponsors</a>
 +
      <ul>
 +
        <li><a href="https://2008.igem.org/Team:University_of_Ottawa/Sponsors#Acedemic_Sponsors">Academic</a></li>
 +
        <li><a href="https://2008.igem.org/Team:University_of_Ottawa/Sponsors#Research_Sponsors">Research</a></li>   
 +
        <li><a href="https://2008.igem.org/Team:University_of_Ottawa/Sponsors#Corporate_Sponsors">Corporate</a></li>       
 +
      </ul>
 +
  </li>
 +
 +
</ul>
 +
<p>&nbsp;</p>
 +
<p>&nbsp;</p>
 +
<script type="text/javascript">
 +
<!--
 +
var MenuBar1 = new Spry.Widget.MenuBar("MenuBar1", {imgDown:"http://www.sysbiolab.uottawa.ca/igem/wiki/SpryAssets/SpryMenuBarDownHover.gif", imgRight:"http://www.sysbiolab.uottawa.ca/igem/wiki/SpryAssets/SpryMenuBarRightHover.gif"});
 +
//-->
 +
</script>
 +
 +
</body>
 +
</html>
 +
 +
__TOC__
__TOC__
==Today in the lab==
==Today in the lab==
Line 4: Line 76:
:'''Ligation'''
:'''Ligation'''
::<li>Spiked my overnight ligation with 1 uL ATP and 1 uL enzyme.
::<li>Spiked my overnight ligation with 1 uL ATP and 1 uL enzyme.
 +
:'''Ligation of Atcre'''
 +
::<li> Began ligation of Chris's Atcre component in ~50 uL reaction
 +
'''Matt'''
 +
:'''Inoculation'''
 +
::<li>Today I took out the inoculated bacteria from Friday and put them in the -4 fridge (PTP2). All of the bacteria grew and the control was clean.
 +
::<li>I also inoculated both BY4742 WT and YPH500 WT for Katy for a Mass Spec test.
 +
:'''PCR'''
 +
::<li>I also performed a PCR cleanup of the 600/01 plasmids after I confirmed them on a Gel. These plasmids will now be ready for integration next week.

Latest revision as of 18:30, 30 July 2008

Untitled Document

 

 


Contents

Today in the lab

Dan

Ligation
  • Spiked my overnight ligation with 1 uL ATP and 1 uL enzyme.
    • Ligation of Atcre
  • Began ligation of Chris's Atcre component in ~50 uL reaction

    • Matt

    Inoculation
  • Today I took out the inoculated bacteria from Friday and put them in the -4 fridge (PTP2). All of the bacteria grew and the control was clean.
  • I also inoculated both BY4742 WT and YPH500 WT for Katy for a Mass Spec test.
    • PCR
  • I also performed a PCR cleanup of the 600/01 plasmids after I confirmed them on a Gel. These plasmids will now be ready for integration next week.
    • Retrieved from "http://2008.igem.org/Team:University_of_Ottawa/19_July_2008"