Team:University of Ottawa/19 July 2008

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__TOC__
__TOC__
==Today in the lab==
==Today in the lab==
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:'''Ligation'''
:'''Ligation'''
::<li>Spiked my overnight ligation with 1 uL ATP and 1 uL enzyme.
::<li>Spiked my overnight ligation with 1 uL ATP and 1 uL enzyme.
-
:''Ligation of Atcre'''
+
:'''Ligation of Atcre'''
::<li> Began ligation of Chris's Atcre component in ~50 uL reaction
::<li> Began ligation of Chris's Atcre component in ~50 uL reaction
 +
'''Matt'''
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:'''Inoculation'''
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::<li>Today I took out the inoculated bacteria from Friday and put them in the -4 fridge (PTP2). All of the bacteria grew and the control was clean.
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::<li>I also inoculated both BY4742 WT and YPH500 WT for Katy for a Mass Spec test.
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:'''PCR'''
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::<li>I also performed a PCR cleanup of the 600/01 plasmids after I confirmed them on a Gel. These plasmids will now be ready for integration next week.

Latest revision as of 18:30, 30 July 2008

Untitled Document

 

 


Contents

Today in the lab

Dan

Ligation
  • Spiked my overnight ligation with 1 uL ATP and 1 uL enzyme.
    • Ligation of Atcre
  • Began ligation of Chris's Atcre component in ~50 uL reaction

    • Matt

    Inoculation
  • Today I took out the inoculated bacteria from Friday and put them in the -4 fridge (PTP2). All of the bacteria grew and the control was clean.
  • I also inoculated both BY4742 WT and YPH500 WT for Katy for a Mass Spec test.
    • PCR
  • I also performed a PCR cleanup of the 600/01 plasmids after I confirmed them on a Gel. These plasmids will now be ready for integration next week.
    • Retrieved from "http://2008.igem.org/Team:University_of_Ottawa/19_July_2008"