Team:University of Ottawa/15 July 2008

From 2008.igem.org

(Difference between revisions)
 
(8 intermediate revisions not shown)
Line 1: Line 1:
 +
{|######Navigational Bar#######
 +
|}
 +
<html xmlns="http://www.w3.org/1999/xhtml">
 +
<head>
 +
<meta http-equiv="Content-Type" content="text/html; charset=UTF-8" />
 +
<title>Untitled Document</title>
 +
<script src="http://www.sysbiolab.uottawa.ca/igem/wiki/SpryAssets/SpryMenuBar.js" type="text/javascript"></script>
 +
<link href="http://www.sysbiolab.uottawa.ca/igem/wiki/SpryAssets/SpryMenuBarHorizontal.css" rel="stylesheet" type="text/css" />
 +
</head>
 +
 +
<body>
 +
 +
<ul id="MenuBar1" class="MenuBarHorizontal">
 +
  <li><a class=MenuBarItemSubmenu href="https://2008.igem.org/Team:University_of_Ottawa">Home</a>
 +
      <ul>
 +
        <li><a href="https://2008.igem.org/Team:University_of_Ottawa#Welcome_to_the_uOttawa_Team_Wiki.21">Welcome</a></li>
 +
        <li><a href="https://2008.igem.org/Team:University_of_Ottawa#Announcements">Announcements</a></li>
 +
      </ul>
 +
  <li><a class=MenuBarItemSubmenu href="https://2008.igem.org/Team:University_of_Ottawa/Team">The Team</a>
 +
      <ul>
 +
        <li><a href="https://2008.igem.org/Team:University_of_Ottawa/Team#Who_we_are">Who We Are</a>
 +
            <ul>
 +
              <li><a href="https://2008.igem.org/Team:University_of_Ottawa/Team#Advisors">Advisors</a></li>
 +
              <li><a href="https://2008.igem.org/Team:University_of_Ottawa/Team#Undergrads">Undergrads</a></li>
 +
            </ul>
 +
        </li>
 +
        <li><a href="https://2008.igem.org/Team:University_of_Ottawa/Team#What_we_did">What We've Done</a></li>
 +
        <li><a href="https://2008.igem.org/Team:University_of_Ottawa/Team#Where_we.27re_from">Where We're From</a></li>
 +
        <li><a href="https://2008.igem.org/Team:University_of_Ottawa/Team#Contact_Us">Contact Us</a></li>
 +
       
 +
      </ul>
 +
  </li>
 +
  <li><a class="MenuBarItemSubmenu" href="https://2008.igem.org/Team:University_of_Ottawa/Project">The Project</a>
 +
      <ul>
 +
        <li><a href="https://2008.igem.org/Team:University_of_Ottawa/Project#Project_Overview">Overview</a></li>
 +
        <li><a href="https://2008.igem.org/Team:University_of_Ottawa/Project#Pulsate_Gene_Expression">Expression</a></li>
 +
        <li><a href="https://2008.igem.org/Team:University_of_Ottawa/Project#Cell-to-Cell_Communication">Communication</a></li>
 +
        <li><a href="https://2008.igem.org/Team:University_of_Ottawa/Project#Oscillatory_Dynamics">Oscillation</a></li>
 +
        <li><a href="https://2008.igem.org/Team:University_of_Ottawa/Project#Applications">Application</a></li>
 +
        <li><a href="https://2008.igem.org/Team:University_of_Ottawa/Project#Journal_Club">Journal Club</a></li>
 +
 +
      </ul>
 +
  </li>
 +
  <li><a href="https://2008.igem.org/Team:University_of_Ottawa/Parts">BioBricks</a></li>
 +
  <li><a href="https://2008.igem.org/Team:University_of_Ottawa/Modeling">Modeling</a></li>
 +
  <li><a class="MenuBarItemSubmenu" href="https://2008.igem.org/Team:University_of_Ottawa/Wet_Lab">Wet Lab</a>
 +
    <ul>
 +
        <li><a href="https://2008.igem.org/Team:University_of_Ottawa/Lab_Protocols">Lab Protocols</a></li>
 +
        <li><a href="https://2008.igem.org/Team:University_of_Ottawa/WetWare">WetWare</a></li>
 +
    </ul>
 +
  <li><a href="https://2008.igem.org/Team:University_of_Ottawa/Notebook">Notebook</a></li>
 +
  <li><a class="MenuBarItemSubmenu" href="https://2008.igem.org/Team:University_of_Ottawa/Sponsors">Sponsors</a>
 +
      <ul>
 +
        <li><a href="https://2008.igem.org/Team:University_of_Ottawa/Sponsors#Acedemic_Sponsors">Academic</a></li>
 +
        <li><a href="https://2008.igem.org/Team:University_of_Ottawa/Sponsors#Research_Sponsors">Research</a></li>   
 +
        <li><a href="https://2008.igem.org/Team:University_of_Ottawa/Sponsors#Corporate_Sponsors">Corporate</a></li>       
 +
      </ul>
 +
  </li>
 +
 +
</ul>
 +
<p>&nbsp;</p>
 +
<p>&nbsp;</p>
 +
<script type="text/javascript">
 +
<!--
 +
var MenuBar1 = new Spry.Widget.MenuBar("MenuBar1", {imgDown:"http://www.sysbiolab.uottawa.ca/igem/wiki/SpryAssets/SpryMenuBarDownHover.gif", imgRight:"http://www.sysbiolab.uottawa.ca/igem/wiki/SpryAssets/SpryMenuBarRightHover.gif"});
 +
//-->
 +
</script>
 +
 +
</body>
 +
</html>
 +
 +
 +
__TOC__
__TOC__
-
==Today on the Lab===
+
==Today in the Lab==
'''Tammy'''
'''Tammy'''
-
:<li>Transformation of XL10 Competent E. coli cells with pDR197:AtCKX2
+
:'''Transformation of XL10 Competent E. coli cells with pDR197:AtCKX2'''
:<li>1 uL Pure pDR197:AtCKX2
:<li>1 uL Pure pDR197:AtCKX2
:<li>1 ul 1:10 dilution pDR197:AtCKX2
:<li>1 ul 1:10 dilution pDR197:AtCKX2
Line 10: Line 83:
:<li>3 LB Ampicillin (50 ug/mL) Plates
:<li>3 LB Ampicillin (50 ug/mL) Plates
:<li>Began Incubation of transformed cells at 1:50 pm.
:<li>Began Incubation of transformed cells at 1:50 pm.
 +
'''Matt'''
 +
:'''Inoculation of 97 - BY4742 cells'''
 +
:<li> I inoculated the 97 BY4742 cells once again for glycerol stock tomorrow
 +
:'''PCR confirmation'''
 +
:<li> Second PCR confirmation with F58, F59 was performed and gave the correct band but also gave another weird band that I don't know what it is.
 +
:'''Digestion of PTP2'''
 +
:<li> A digestion was performed on the PTP2 amplification product to create sticky ends using BamHI and xhoI and I left it in the PCR machine overnight.
 +
'''Chris'''
 +
:'''Digestion of AtCRE'''
 +
::<li> AtCRE was digested using EagI at 37 C for about one hour
 +
::<li> the result was run on a 1% gel, which showed two bands, both of which were expected
 +
:'''Gel Extraction of AtCRE'''
 +
::<li> the AtCRE was extracted from the gel using the gel extraction kit and protocol
 +
::<li> unfortunately, the final AtCRE sample was vacuumed up with the other waste by accident
 +
:'''Digestion of AtCRE'''
 +
::<li> AtCRE was digested again using the same protocols as above, except that it was run on the "digest and denature" program on the thermal cycler. It was left overnight.
 +
'''Dan'''
 +
:'''Gel of overnight PCR'''
 +
::<li>Sample 0B did not show up on the gel, and was thus considered unsuccessful. This information was coherent with results from the absorbance measurements.
 +
:'''PCR at 35 cycles'''
 +
::<li>PCR reaction of 0B was run overnight at 35 cycles instead of 29.

Latest revision as of 18:31, 30 July 2008

Untitled Document

 

 


Contents

Today in the Lab

Tammy

Transformation of XL10 Competent E. coli cells with pDR197:AtCKX2
  • 1 uL Pure pDR197:AtCKX2
  • 1 ul 1:10 dilution pDR197:AtCKX2
  • 1 ul 1:100 dilution pDR197:AtCKX2
  • 100 ul XL10 E.Coli per reaction
  • 900 ul LB Broth per reaction
  • 3 LB Ampicillin (50 ug/mL) Plates
  • Began Incubation of transformed cells at 1:50 pm.

    • Matt

    Inoculation of 97 - BY4742 cells
  • I inoculated the 97 BY4742 cells once again for glycerol stock tomorrow
    • PCR confirmation
  • Second PCR confirmation with F58, F59 was performed and gave the correct band but also gave another weird band that I don't know what it is.
    • Digestion of PTP2
  • A digestion was performed on the PTP2 amplification product to create sticky ends using BamHI and xhoI and I left it in the PCR machine overnight.

    • Chris

    Digestion of AtCRE
  • AtCRE was digested using EagI at 37 C for about one hour
  • the result was run on a 1% gel, which showed two bands, both of which were expected
    • Gel Extraction of AtCRE
  • the AtCRE was extracted from the gel using the gel extraction kit and protocol
  • unfortunately, the final AtCRE sample was vacuumed up with the other waste by accident
    • Digestion of AtCRE
  • AtCRE was digested again using the same protocols as above, except that it was run on the "digest and denature" program on the thermal cycler. It was left overnight.

    • Dan

    Gel of overnight PCR
  • Sample 0B did not show up on the gel, and was thus considered unsuccessful. This information was coherent with results from the absorbance measurements.
    • PCR at 35 cycles
  • PCR reaction of 0B was run overnight at 35 cycles instead of 29.
    • Retrieved from "http://2008.igem.org/Team:University_of_Ottawa/15_July_2008"