Team:University of Ottawa/11 July 2008

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__TOC__
__TOC__
==Today in the Lab==
==Today in the Lab==
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:'''PCR confirmation'''
:'''PCR confirmation'''
::<li> PCR confirmation of 97 plasmid showed up nicely on the gel, confirming that the integration was a success.
::<li> PCR confirmation of 97 plasmid showed up nicely on the gel, confirming that the integration was a success.
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'''Chris'''
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:'''Confirmation of AtCRE'''
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::<li> ran the 1% gel at 80 V for about forty minutes
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::<li> only primer bands showed after this time period
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::<li> it was suggested that phusion polymerase may not have been added before PCR, so 1 uL of phusion was added to the samples and phusion PCR was re-initiated
 +
::<li> following PCR, the results were run on a gel with the same results as above.

Latest revision as of 18:32, 30 July 2008

Untitled Document

 

 


Contents

Today in the Lab

Matt

PTP2 Digestion
  • Ran two digestion's of the PTP2 in parallel with one another: AatII/NcoI, EcoRV/NcoI. Neither Gel gave proper band sizes.
    • PCR confirmation
  • PCR confirmation of 97 plasmid showed up nicely on the gel, confirming that the integration was a success.

    • Chris

    Confirmation of AtCRE
  • ran the 1% gel at 80 V for about forty minutes
  • only primer bands showed after this time period
  • it was suggested that phusion polymerase may not have been added before PCR, so 1 uL of phusion was added to the samples and phusion PCR was re-initiated
  • following PCR, the results were run on a gel with the same results as above.
    • Retrieved from "http://2008.igem.org/Team:University_of_Ottawa/11_July_2008"