"
Team:University of Ottawa/26 July 2008
From 2008.igem.org
(Difference between revisions)
(→Today in the Lab) |
|||
| Line 73: | Line 73: | ||
'''Tammy''' | '''Tammy''' | ||
:<li> '''Digestion of pSSA14''' | :<li> '''Digestion of pSSA14''' | ||
| + | <TABLE BORDER=2> | ||
| + | <TR> <TD>'''Reaction components'''</TD><TD>'''1x Vol (μl)'''</TD> </TR> | ||
| + | <TR> <TD>10X Reaction buffer</TD><TD>2.5</TD> </TR> | ||
| + | <TR> <TD>10mM each dNTP</TD><TD>0.5</TD> </TR> | ||
| + | <TR> <TD>Forward primer (10pmol/μl)</TD><TD>1.25</TD> </TR> | ||
| + | <TR> <TD>Reverse primer (10pmol/μl)</TD><TD>1.25</TD> </TR> | ||
| + | <TR> <TD>DNA template</TD><TD>2</TD> </TR> | ||
| + | <TR> <TD>Taq polymerase</TD><TD>0.5</TD> </TR> | ||
| + | <TR> <TD>Filter sterile ddH2O</TD><TD>17</TD> </TR> | ||
| + | <TR> <TD>Total</TD><TD>25</TD> </TR> | ||
| + | </TABLE> | ||
| + | |||
| + | |||
:<li> '''Digestion of OB''' | :<li> '''Digestion of OB''' | ||
Revision as of 18:37, 30 July 2008
Contents |
Today in the Lab
Tammy
- Digestion of pSSA14
| Reaction components | 1x Vol (μl) |
| 10X Reaction buffer | 2.5 |
| 10mM each dNTP | 0.5 |
| Forward primer (10pmol/μl) | 1.25 |
| Reverse primer (10pmol/μl) | 1.25 |
| DNA template | 2 |
| Taq polymerase | 0.5 |
| Filter sterile ddH2O | 17 |
| Total | 25 |
- Digestion of OB
