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Team:KULeuven/30 July 2008
From 2008.igem.org
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*Letting ''Xba''I doing its job during the night, gave a positive outcome. These parts (B0015, J23032) were then cut with ''Eco''RI, separated on gel and purified. The following ligations were made: C0040+B0015, J23109+J23032, I712074+J23032, E0022+B0015. | *Letting ''Xba''I doing its job during the night, gave a positive outcome. These parts (B0015, J23032) were then cut with ''Eco''RI, separated on gel and purified. The following ligations were made: C0040+B0015, J23109+J23032, I712074+J23032, E0022+B0015. | ||
*R0084 + B0032 were separated on a gel, but no fragment of 145bp was observed => so no ligation | *R0084 + B0032 were separated on a gel, but no fragment of 145bp was observed => so no ligation | ||
| - | *Fluid cultures were prepared with the following parts: C0062, R0062, P1010, R0011, C0060, I712022. | + | *Fluid cultures were prepared with the following parts: C0062, R0062, P1010, R0011, C0060, I712022, C0012, B0015, F1610. |
| - | *New digests with ''Xba''I were made with: B0015, B0032, J23022, J23032. | + | *New digests with ''Xba''I were made with: B0015, B0032, J23022, J23032, F1610 |
| + | *We punched the DNA of the memory and checked the concentration with the nanodrop. Despite the doubtfull results we did try to electroporate Top10 cells together with these parts. | ||
=== Dry Lab === | === Dry Lab === | ||
Revision as of 13:16, 31 July 2008
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Contents |
Lab Work
Wet Lab
- Letting XbaI doing its job during the night, gave a positive outcome. These parts (B0015, J23032) were then cut with EcoRI, separated on gel and purified. The following ligations were made: C0040+B0015, J23109+J23032, I712074+J23032, E0022+B0015.
- R0084 + B0032 were separated on a gel, but no fragment of 145bp was observed => so no ligation
- Fluid cultures were prepared with the following parts: C0062, R0062, P1010, R0011, C0060, I712022, C0012, B0015, F1610.
- New digests with XbaI were made with: B0015, B0032, J23022, J23032, F1610
- We punched the DNA of the memory and checked the concentration with the nanodrop. Despite the doubtfull results we did try to electroporate Top10 cells together with these parts.
Dry Lab
I worked further on the detailed descriptions of the project. More attention will go to figures and easy reading.
Tomorrow, primers for checking the EnvZ::KmR mutation will be completed with the help of Sigrid. The Restriction Cut Sites seem not be on the same place as indicated in the Japanese paper.
Modeling
All the CellDesigner and Matlab models as the model pictures are updated. Everything is now ready for some extra simulations and a sensitivity analysis for the different models...
Wiki
New drop-down navigation bar has been posted, allowing subsubmenu's that toggle. Hacks have been added to conform with the most important browsers (IE, Mozilla, Safari). If any trouble occur, mail me (avdmeers).
Some bugs have been fixed in notebook.
Started making div stuff for homepage in case Imperial College's style is wanted.
Remarks
Tippenvulwedstrijd:
Hanne: 1'36"
Maarten: 1'42"
Stefanie: 1'49"
