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Template:Team:UC Berkeley/Notebook/Molly construction
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| + | == Molly's Construction Files == | ||
| + | |||
| + | [[Team:UC_Berkeley/Notebook/Molly_Allen|Molly's Notebook]]<br> | ||
| + | |||
<pre> | <pre> | ||
Construction of {xis!} Biobrick Part K112200 | Construction of {xis!} Biobrick Part K112200 | ||
| Line 6: | Line 10: | ||
---------------------------------------- | ---------------------------------------- | ||
mea001 Forward Biobricking of {xis!} ccataGAATTCatgAGATCTatgtacttgacacttcaggag | mea001 Forward Biobricking of {xis!} ccataGAATTCatgAGATCTatgtacttgacacttcaggag | ||
| - | mea002 Reverse Biobricking of {xis!} | + | mea002 Reverse Biobricking of {xis!} cgttaGGATCCtcatgacttcgccttcttccc |
</pre> | </pre> | ||
| Line 16: | Line 20: | ||
---------------------------------------- | ---------------------------------------- | ||
mea003 Forward Biobricking of {<xis!} ccataGAATTCatgAGATCTtacttgacacttcaggagtg | mea003 Forward Biobricking of {<xis!} ccataGAATTCatgAGATCTtacttgacacttcaggagtg | ||
| - | mea002 Reverse Biobricking of {xis!} | + | mea002 Reverse Biobricking of {xis!} cgttaGGATCCtcatgacttcgccttcttccc |
</pre> | </pre> | ||
| - | |||
<pre> | <pre> | ||
| Line 29: | Line 32: | ||
mea004 Reverse Biobricking of {xis>} cgttaGGATCCtgacttcgccttcttcccatttc | mea004 Reverse Biobricking of {xis>} cgttaGGATCCtgacttcgccttcttcccatttc | ||
</pre> | </pre> | ||
| - | |||
<pre> | <pre> | ||
| Line 40: | Line 42: | ||
mea004 Reverse Biobricking of {xis>} cgttaGGATCCtgacttcgccttcttcccatttc | mea004 Reverse Biobricking of {xis>} cgttaGGATCCtgacttcgccttcttcccatttc | ||
</pre> | </pre> | ||
| - | |||
<pre> | <pre> | ||
| Line 48: | Line 49: | ||
Product is pBca1256-K112204 | Product is pBca1256-K112204 | ||
---------------------------------------- | ---------------------------------------- | ||
| - | mea017 Forward Biobricking of {a~xis!} | + | mea017 Forward Biobricking of {a~xis!}ccagtGAATTCatgAGATCTtgcgatgtacttgacacttcagg |
| - | mea002 Reverse Biobricking of {xis!} | + | mea002 Reverse Biobricking of {xis!} cgttaGGATCCtcatgacttcgccttcttccc |
</pre> | </pre> | ||
| - | |||
<pre> | <pre> | ||
| Line 59: | Line 59: | ||
Product is pBca1256-K112205 | Product is pBca1256-K112205 | ||
---------------------------------------- | ---------------------------------------- | ||
| - | mea018 Forward Biobricking of {rbs.xis!} | + | mea018 Forward Biobricking of {rbs.xis!}ccagtGAATTCatgAGATCTtaattgcggagactttgcgatg |
| - | mea002 Reverse Biobricking of {xis!} | + | mea002 Reverse Biobricking of {xis!} cgttaGGATCCtcatgacttcgccttcttccc |
</pre> | </pre> | ||
| - | |||
<pre> | <pre> | ||
| Line 71: | Line 70: | ||
---------------------------------------- | ---------------------------------------- | ||
mea005 Forward Biobricking of {int!} ccataGAATTCatgAGATCTatgggaagaaggcgaagtcatg | mea005 Forward Biobricking of {int!} ccataGAATTCatgAGATCTatgggaagaaggcgaagtcatg | ||
| - | mea006 Reverse Biobricking of {int!} | + | mea006 Reverse Biobricking of {int!} cgttaGGATCCttatttgatttcaattttgtcccactccc |
</pre> | </pre> | ||
| - | |||
<pre> | <pre> | ||
| Line 82: | Line 80: | ||
---------------------------------------- | ---------------------------------------- | ||
mea007 Forward Biobricking of {<int!} ccataGAATTCatgAGATCTggaagaaggcgaagtcatgagc | mea007 Forward Biobricking of {<int!} ccataGAATTCatgAGATCTggaagaaggcgaagtcatgagc | ||
| - | mea006 Reverse Biobricking of {int!} | + | mea006 Reverse Biobricking of {int!} cgttaGGATCCttatttgatttcaattttgtcccactccc |
</pre> | </pre> | ||
| - | |||
<pre> | <pre> | ||
| Line 95: | Line 92: | ||
mea008 Reverse Biobricking of {int>} cgttaGGATCCtttgatttcaattttgtcccactccc | mea008 Reverse Biobricking of {int>} cgttaGGATCCtttgatttcaattttgtcccactccc | ||
</pre> | </pre> | ||
| - | |||
<pre> | <pre> | ||
| Line 106: | Line 102: | ||
mea008 Reverse Biobricking of {int>} cgttaGGATCCtttgatttcaattttgtcccactccc | mea008 Reverse Biobricking of {int>} cgttaGGATCCtttgatttcaattttgtcccactccc | ||
</pre> | </pre> | ||
| - | |||
<pre> | <pre> | ||
| Line 115: | Line 110: | ||
---------------------------------------- | ---------------------------------------- | ||
mea019 Forward Biobricking of {a~int!}ccataGAATTCatgAGATCTagaaatgggaagaaggcgaag | mea019 Forward Biobricking of {a~int!}ccataGAATTCatgAGATCTagaaatgggaagaaggcgaag | ||
| - | mea006 Reverse Biobricking of {int!} | + | mea006 Reverse Biobricking of {int!} cgttaGGATCCttatttgatttcaattttgtcccactccc |
</pre> | </pre> | ||
| - | |||
<pre> | <pre> | ||
| Line 126: | Line 120: | ||
---------------------------------------- | ---------------------------------------- | ||
mea020 Forward Biobricking of {rbs.int!}ccataGAATTCatgAGATCTttttgaagaggatcagaaatggg | mea020 Forward Biobricking of {rbs.int!}ccataGAATTCatgAGATCTttttgaagaggatcagaaatggg | ||
| - | mea006 Reverse Biobricking of {int!} | + | mea006 Reverse Biobricking of {int!} cgttaGGATCCttatttgatttcaattttgtcccactccc |
</pre> | </pre> | ||
| - | |||
<pre> | <pre> | ||
| Line 137: | Line 130: | ||
---------------------------------------- | ---------------------------------------- | ||
mea009 Forward Biobricking of {ihfA!}ccataGAATTCatgAGATCTatggcgcttacaaaagctgaaatg | mea009 Forward Biobricking of {ihfA!}ccataGAATTCatgAGATCTatggcgcttacaaaagctgaaatg | ||
| - | mea010 Reverse Biobricking of {ihfA!} | + | mea010 Reverse Biobricking of {ihfA!} cgttaGGATCCttactcgtctttgggcgaagcg |
</pre> | </pre> | ||
| - | |||
<pre> | <pre> | ||
| Line 148: | Line 140: | ||
---------------------------------------- | ---------------------------------------- | ||
mea011 Forward Biobricking of {<ihfA!}ccataGAATTCatgAGATCTgcgcttacaaaagctgaaatgtc | mea011 Forward Biobricking of {<ihfA!}ccataGAATTCatgAGATCTgcgcttacaaaagctgaaatgtc | ||
| - | mea010 Reverse Biobricking of {ihfA!} | + | mea010 Reverse Biobricking of {ihfA!} cgttaGGATCCttactcgtctttgggcgaagcg |
</pre> | </pre> | ||
| - | |||
<pre> | <pre> | ||
| Line 161: | Line 152: | ||
mea012 Reverse Biobricking of {ihfA>} cgttaGGATCCctcgtctttgggcgaagcg | mea012 Reverse Biobricking of {ihfA>} cgttaGGATCCctcgtctttgggcgaagcg | ||
</pre> | </pre> | ||
| - | |||
<pre> | <pre> | ||
| Line 172: | Line 162: | ||
mea012 Reverse Biobricking of {ihfA>} cgttaGGATCCctcgtctttgggcgaagcg | mea012 Reverse Biobricking of {ihfA>} cgttaGGATCCctcgtctttgggcgaagcg | ||
</pre> | </pre> | ||
| - | |||
<pre> | <pre> | ||
| Line 181: | Line 170: | ||
---------------------------------------- | ---------------------------------------- | ||
mea021 Forward Biobricking of {a~ihfA!}ccataGAATTCatgAGATCTacctatggcgcttacaaaagc | mea021 Forward Biobricking of {a~ihfA!}ccataGAATTCatgAGATCTacctatggcgcttacaaaagc | ||
| - | mea010 Reverse Biobricking of {ihfA!} | + | mea010 Reverse Biobricking of {ihfA!} cgttaGGATCCttactcgtctttgggcgaagcg |
</pre> | </pre> | ||
| - | |||
<pre> | <pre> | ||
| Line 191: | Line 179: | ||
Product is pBca1256-K112218 | Product is pBca1256-K112218 | ||
---------------------------------------- | ---------------------------------------- | ||
| - | mea022 Forward Biobricking of {rbs.ihfA!} | + | mea022 Forward Biobricking of {rbs.ihfA!}ccataGAATTCatgAGATCTtcattgagggattgaacctatggcgc |
| - | mea010 Reverse Biobricking of {ihfA!} | + | mea010 Reverse Biobricking of {ihfA!} cgttaGGATCCttactcgtctttgggcgaagcg |
</pre> | </pre> | ||
| - | |||
<pre> | <pre> | ||
| Line 202: | Line 189: | ||
Product is pBca1256-K112219 | Product is pBca1256-K112219 | ||
---------------------------------------- | ---------------------------------------- | ||
| - | mea013 Forward Biobricking of {ihfB!} | + | mea013 Forward Biobricking of {ihfB!} ccataGAATTCatgAGATCTatgaccaagtcagaattgatagaaagacttgccaccc |
mea014 Reverse Biobricking of {ihfB!} cgttaGGATCCttaaccgtaaatattggcgcgatcg | mea014 Reverse Biobricking of {ihfB!} cgttaGGATCCttaaccgtaaatattggcgcgatcg | ||
</pre> | </pre> | ||
| - | |||
<pre> | <pre> | ||
| Line 213: | Line 199: | ||
Product is pBca1256-K112220 | Product is pBca1256-K112220 | ||
---------------------------------------- | ---------------------------------------- | ||
| - | mea015 Forward Biobricking of {<ihfB!} | + | mea015 Forward Biobricking of {<ihfB!} ccataGAATTCatgAGATCTaccaagtcagaattgatagaaagacttgccaccc |
mea014 Reverse Biobricking of {ihfB!} cgttaGGATCCttaaccgtaaatattggcgcgatcg | mea014 Reverse Biobricking of {ihfB!} cgttaGGATCCttaaccgtaaatattggcgcgatcg | ||
</pre> | </pre> | ||
| - | |||
<pre> | <pre> | ||
| Line 224: | Line 209: | ||
Product is pBca1256-K112221 | Product is pBca1256-K112221 | ||
---------------------------------------- | ---------------------------------------- | ||
| - | mea013 Forward Biobricking of {ihfB!} | + | mea013 Forward Biobricking of {ihfB!} ccataGAATTCatgAGATCTatgaccaagtcagaattgatagaaagacttgccaccc |
mea016 Reverse Biobricking of {ihfB>} cgttaGGATCCaccgtaaatattggcgcgatc | mea016 Reverse Biobricking of {ihfB>} cgttaGGATCCaccgtaaatattggcgcgatc | ||
</pre> | </pre> | ||
| - | |||
<pre> | <pre> | ||
| Line 235: | Line 219: | ||
Product is pBca1256-K112222 | Product is pBca1256-K112222 | ||
---------------------------------------- | ---------------------------------------- | ||
| - | mea015 Forward Biobricking of {<ihfB!} | + | mea015 Forward Biobricking of {<ihfB!} ccataGAATTCatgAGATCTaccaagtcagaattgatagaaagacttgccaccc |
mea016 Reverse Biobricking of {ihfB>} cgttaGGATCCaccgtaaatattggcgcgatc | mea016 Reverse Biobricking of {ihfB>} cgttaGGATCCaccgtaaatattggcgcgatc | ||
</pre> | </pre> | ||
| Line 241: | Line 225: | ||
<pre> | <pre> | ||
Construction of {a~ihfB!} Biobrick Part K112223 | Construction of {a~ihfB!} Biobrick Part K112223 | ||
| - | PCR mea023/mea014 on MG1655 ( | + | PCR mea023/mea014 on MG1655 (320 bp, EcoRI/BamHI/DpnI) |
Sub into pBca1256 (EcoRI/BamHI, 2472+910, L) | Sub into pBca1256 (EcoRI/BamHI, 2472+910, L) | ||
Product is pBca1256-K112223 | Product is pBca1256-K112223 | ||
| Line 248: | Line 232: | ||
mea014 Reverse Biobricking of {ihfB!} cgttaGGATCCttaaccgtaaatattggcgcgatcg | mea014 Reverse Biobricking of {ihfB!} cgttaGGATCCttaaccgtaaatattggcgcgatcg | ||
</pre> | </pre> | ||
| - | |||
<pre> | <pre> | ||
| Line 259: | Line 242: | ||
mea014 Reverse Biobricking of {ihfB!} cgttaGGATCCttaaccgtaaatattggcgcgatcg | mea014 Reverse Biobricking of {ihfB!} cgttaGGATCCttaaccgtaaatattggcgcgatcg | ||
</pre> | </pre> | ||
| + | |||
| + | <pre> | ||
| + | Construction of {ompT>} Biobrick Part K112225 | ||
| + | Wobble PCR mea025/mea026 (97 bp, EcoRI/BamHI/DpnI) | ||
| + | Sub into pBca1256 (EcoRI/BamHI, 2472+910, L) | ||
| + | Product is pBca1256-K112225 | ||
| + | ---------------------------------------- | ||
| + | mea025 Forward Biobricking of {ompT>} cgataGAATTCatgAGATCTatgcgggcgaaactcctaggaatagtcctgacaacccctatcg | ||
| + | mea026 Reverse Biobricking of {ompT>} cgttaGGATCCcgtcgacgcaaaagagctgatcgcgataggggttgtcaggactattc | ||
| + | </pre> | ||
| + | |||
| + | <pre> | ||
| + | Construction of {a~ompT} Biobrick Part K112226 | ||
| + | Wobble PCR mea027/mea026 (101 bp, EcoRI/BamHI/DpnI) | ||
| + | Sub into pBca1256 (EcoRI/BamHI, 2472+910, L) | ||
| + | Product is pBca1256-K112226 | ||
| + | ---------------------------------------- | ||
| + | mea027 Forward Biobricking of {a~ompT} cgataGAATTCatgAGATCTacatatgcgggcgaaactcctaggaatagtcctgacaacccctatc | ||
| + | mea026 Reverse Biobricking of {ompT>} cgttaGGATCCcgtcgacgcaaaagagctgatcgcgataggggttgtcaggactattc | ||
| + | </pre> | ||
| + | |||
| + | <pre> | ||
| + | Construction of {rbs.ompT} Biobrick Part K112227 | ||
| + | Wobble PCR mea028/mea026 (114 bp, EcoRI/BamHI/DpnI) | ||
| + | Sub into pBca1256 (EcoRI/BamHI, 2472+910, L) | ||
| + | Product is pBca1256-K112227 | ||
| + | ---------------------------------------- | ||
| + | mea028 Forward Biobricking of {rbs.ompT}cgataGAATTCatgAGATCTaagaaggagatatacatatgcgggcgaaactcctaggaatagtcctgacaacccc | ||
| + | mea026 Reverse Biobricking of {ompT>} cgttaGGATCCcgtcgacgcaaaagagctgatcgcgataggggttgtcaggactattc | ||
| + | </pre> | ||
| + | |||
| + | <pre> | ||
| + | Construction of {pelB>} Biobrick Part K112228 | ||
| + | Wobble PCR mea029/mea030 (97 bp, EcoRI/BamHI/DpnI) | ||
| + | Sub into pBca1256 (EcoRI/BamHI, 2472+910, L) | ||
| + | Product is pBca1256-K112228 | ||
| + | ---------------------------------------- | ||
| + | mea029 Forward Biobricking of {pelB>} cgataGAATTCatgAGATCTatgaaatacctgctgccgaccgctgctgctggtctgctgctcc | ||
| + | mea030 Reverse Biobricking of {pelB>} cgttaGGATCCggccatcgccggctgggcagcgaggagcagcagaccagcagcagcgg | ||
| + | </pre> | ||
| + | |||
| + | <pre> | ||
| + | Construction of {a~pelB} Biobrick Part K112229 | ||
| + | Wobble PCR mea031/mea030 (101 bp, EcoRI/BamHI/DpnI) | ||
| + | Sub into pBca1256 (EcoRI/BamHI, 2472+910, L) | ||
| + | Product is pBca1256-K112229 | ||
| + | ---------------------------------------- | ||
| + | mea031 Forward Biobricking of {a~pelB} cgataGAATTCatgAGATCTacatatgaaatacctgctgccgaccgctgctgctggtctgctg | ||
| + | mea030 Reverse Biobricking of {pelB>} cgttaGGATCCggccatcgccggctgggcagcgaggagcagcagaccagcagcagcgg | ||
| + | </pre> | ||
| + | |||
| + | <pre> | ||
| + | Construction of {rbs.pelB} Biobrick Part K112230 | ||
| + | Wobble PCR mea032/mea030 (116 bp, EcoRI/BamHI/DpnI) | ||
| + | Sub into pBca1256 (EcoRI/BamHI, 2472+910, L) | ||
| + | Product is pBca1256-K112230 | ||
| + | ---------------------------------------- | ||
| + | mea032 Forward Biobricking of {rbs.pelB} cgataGAATTCatgAGATCTttaagaaggagatatacatatgaaatacctgctgccgaccgctgctgctggtctgctg | ||
| + | mea030 Reverse Biobricking of {pelB>} cgttaGGATCCggccatcgccggctgggcagcgaggagcagcagaccagcagcagcgg | ||
| + | </pre> | ||
| + | |||
| + | <pre> | ||
| + | Construction of {rbs.xis.int!} Biobrick Part K112234 | ||
| + | PCR mea018/mea006 on pAH57 (1317 bp, EcoRI/BamHI/DpnI) | ||
| + | Sub into pBca1256 (EcoRI/BamHI, 2472+910, L) | ||
| + | Product is pBca1256-K112234 | ||
| + | ---------------------------------------- | ||
| + | mea018 Forward Biobricking of {rbs.xis!}ccagtGAATTCatgAGATCTtaattgcggagactttgcgatg | ||
| + | mea006 Reverse Biobricking of {int!} cgttaGGATCCttatttgatttcaattttgtcccactccc | ||
| + | </pre> | ||
| + | |||
[[Team:UC_Berkeley/Notebook/Molly_Allen|Molly's Notebook]]<br> | [[Team:UC_Berkeley/Notebook/Molly_Allen|Molly's Notebook]]<br> | ||
Latest revision as of 00:06, 4 August 2008
Molly's Construction Files
Construction of {xis!} Biobrick Part K112200
PCR mea001/mea002 on pAH57 (230 bp, EcoRI/BamHI/DpnI)
Sub into pBca1256 (EcoRI/BamHI, 2472+910, L)
Product is pBca1256-K112200
----------------------------------------
mea001 Forward Biobricking of {xis!} ccataGAATTCatgAGATCTatgtacttgacacttcaggag
mea002 Reverse Biobricking of {xis!} cgttaGGATCCtcatgacttcgccttcttccc
Construction of {<xis!} Biobrick Part K112201
PCR mea003/mea002 on pAH57 (227 bp, EcoRI/BamHI/DpnI)
Sub into pBca1256 (EcoRI/BamHI, 2472+910, L)
Product is pBca1256-K112201
----------------------------------------
mea003 Forward Biobricking of {<xis!} ccataGAATTCatgAGATCTtacttgacacttcaggagtg
mea002 Reverse Biobricking of {xis!} cgttaGGATCCtcatgacttcgccttcttccc
Construction of {xis>} Biobrick Part K112202
PCR mea001/mea004 on pAH57 (227 bp, EcoRI/BamHI/DpnI)
Sub into pBca1256 (EcoRI/BamHI, 2472+910, L)
Product is pBca1256-K112202
----------------------------------------
mea001 Forward Biobricking of {xis!} ccataGAATTCatgAGATCTatgtacttgacacttcaggag
mea004 Reverse Biobricking of {xis>} cgttaGGATCCtgacttcgccttcttcccatttc
Construction of {<xis>} Biobrick Part K112203
PCR mea003/mea004 on pAH57 (224 bp, EcoRI/BamHI/DpnI)
Sub into pBca1256 (EcoRI/BamHI, 2472+910, L)
Product is pBca1256-K112203
----------------------------------------
mea003 Forward Biobricking of {<xis!} ccataGAATTCatgAGATCTtacttgacacttcaggagtg
mea004 Reverse Biobricking of {xis>} cgttaGGATCCtgacttcgccttcttcccatttc
Construction of {a~xis!} Biobrick Part K112204
PCR mea017/mea002 on pAH57 (234 bp, EcoRI/BamHI/DpnI)
Sub into pBca1256 (EcoRI/BamHI, 2472+910, L)
Product is pBca1256-K112204
----------------------------------------
mea017 Forward Biobricking of {a~xis!}ccagtGAATTCatgAGATCTtgcgatgtacttgacacttcagg
mea002 Reverse Biobricking of {xis!} cgttaGGATCCtcatgacttcgccttcttccc
Construction of {rbs.xis!} Biobrick Part K112205
PCR mea018/mea002 on pAH57 (249 bp, EcoRI/BamHI/DpnI)
Sub into pBca1256 (EcoRI/BamHI, 2472+910, L)
Product is pBca1256-K112205
----------------------------------------
mea018 Forward Biobricking of {rbs.xis!}ccagtGAATTCatgAGATCTtaattgcggagactttgcgatg
mea002 Reverse Biobricking of {xis!} cgttaGGATCCtcatgacttcgccttcttccc
Construction of {int!} Biobrick Part K112207
PCR mea005/mea006 on pAH57 (1102 bp, EcoRI/BamHI/DpnI)
Sub into pBca1256 (EcoRI/BamHI, 2472+910, L)
Product is pBca1256-K112207
----------------------------------------
mea005 Forward Biobricking of {int!} ccataGAATTCatgAGATCTatgggaagaaggcgaagtcatg
mea006 Reverse Biobricking of {int!} cgttaGGATCCttatttgatttcaattttgtcccactccc
Construction of {<int!} Biobrick Part K112208
PCR mea007/mea006 on pAH57 (1099 bp, EcoRI/BamHI/DpnI)
Sub into pBca1256 (EcoRI/BamHI, 2472+910, L)
Product is pBca1256-K112208
----------------------------------------
mea007 Forward Biobricking of {<int!} ccataGAATTCatgAGATCTggaagaaggcgaagtcatgagc
mea006 Reverse Biobricking of {int!} cgttaGGATCCttatttgatttcaattttgtcccactccc
Construction of {int>} Biobrick Part K112209
PCR mea005/mea008 on pAH57 (1099 bp, EcoRI/BamHI/DpnI)
Sub into pBca1256 (EcoRI/BamHI, 2472+910, L)
Product is pBca1256-K112209
----------------------------------------
mea005 Forward Biobricking of {int!} ccataGAATTCatgAGATCTatgggaagaaggcgaagtcatg
mea008 Reverse Biobricking of {int>} cgttaGGATCCtttgatttcaattttgtcccactccc
Construction of {<int>} Biobrick Part K112210
PCR mea007/mea008 on pAH57 (1096 bp, EcoRI/BamHI/DpnI)
Sub into pBca1256 (EcoRI/BamHI, 2472+910, L)
Product is pBca1256-K112210
----------------------------------------
mea007 Forward Biobricking of {<int!} ccataGAATTCatgAGATCTggaagaaggcgaagtcatgagc
mea008 Reverse Biobricking of {int>} cgttaGGATCCtttgatttcaattttgtcccactccc
Construction of {a~int!} Biobrick Part K112211
PCR mea019/mea006 on pAH57 (1106 bp, EcoRI/BamHI/DpnI)
Sub into pBca1256 (EcoRI/BamHI, 2472+910, L)
Product is pBca1256-K112211
----------------------------------------
mea019 Forward Biobricking of {a~int!}ccataGAATTCatgAGATCTagaaatgggaagaaggcgaag
mea006 Reverse Biobricking of {int!} cgttaGGATCCttatttgatttcaattttgtcccactccc
Construction of {rbs.int!} Biobrick Part K112212
PCR mea020/mea006 on pAH57 (1120 bp, EcoRI/BamHI/DpnI)
Sub into pBca1256 (EcoRI/BamHI, 2472+910, L)
Product is pBca1256-K112212
----------------------------------------
mea020 Forward Biobricking of {rbs.int!}ccataGAATTCatgAGATCTttttgaagaggatcagaaatggg
mea006 Reverse Biobricking of {int!} cgttaGGATCCttatttgatttcaattttgtcccactccc
Construction of {ihfA!} Biobrick Part K112213
PCR mea009/mea010 on MG1655 (331 bp, EcoRI/BamHI/DpnI)
Sub into pBca1256 (EcoRI/BamHI, 2472+910, L)
Product is pBca1256-K112213
----------------------------------------
mea009 Forward Biobricking of {ihfA!}ccataGAATTCatgAGATCTatggcgcttacaaaagctgaaatg
mea010 Reverse Biobricking of {ihfA!} cgttaGGATCCttactcgtctttgggcgaagcg
Construction of {<ihfA!} Biobrick Part K112214
PCR mea011/mea010 on MG1655 (328 bp, EcoRI/BamHI/DpnI)
Sub into pBca1256 (EcoRI/BamHI, 2472+910, L)
Product is pBca1256-K112214
----------------------------------------
mea011 Forward Biobricking of {<ihfA!}ccataGAATTCatgAGATCTgcgcttacaaaagctgaaatgtc
mea010 Reverse Biobricking of {ihfA!} cgttaGGATCCttactcgtctttgggcgaagcg
Construction of {ihfA>} Biobrick Part K112215
PCR mea009/mea012 on MG1655 (328 bp, EcoRI/BamHI/DpnI)
Sub into pBca1256 (EcoRI/BamHI, 2472+910, L)
Product is pBca1256-K112215
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mea009 Forward Biobricking of {ihfA!}ccataGAATTCatgAGATCTatggcgcttacaaaagctgaaatg
mea012 Reverse Biobricking of {ihfA>} cgttaGGATCCctcgtctttgggcgaagcg
Construction of {<ihfA>} Biobrick Part K112216
PCR mea011/mea012 on MG1655 (325 bp, EcoRI/BamHI/DpnI)
Sub into pBca1256 (EcoRI/BamHI, 2472+910, L)
Product is pBca1256-K112216
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mea011 Forward Biobricking of {<ihfA!}ccataGAATTCatgAGATCTgcgcttacaaaagctgaaatgtc
mea012 Reverse Biobricking of {ihfA>} cgttaGGATCCctcgtctttgggcgaagcg
Construction of {a~ihfA!} Biobrick Part K112217
PCR mea021/mea010 on MG1655 (335 bp, EcoRI/BamHI/DpnI)
Sub into pBca1256 (EcoRI/BamHI, 2472+910, L)
Product is pBca1256-K112217
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mea021 Forward Biobricking of {a~ihfA!}ccataGAATTCatgAGATCTacctatggcgcttacaaaagc
mea010 Reverse Biobricking of {ihfA!} cgttaGGATCCttactcgtctttgggcgaagcg
Construction of {rbs.ihfA!} Biobrick Part K112218
PCR mea022/mea010 on MG1655 (350 bp, EcoRI/BamHI/DpnI)
Sub into pBca1256 (EcoRI/BamHI, 2472+910, L)
Product is pBca1256-K112218
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mea022 Forward Biobricking of {rbs.ihfA!}ccataGAATTCatgAGATCTtcattgagggattgaacctatggcgc
mea010 Reverse Biobricking of {ihfA!} cgttaGGATCCttactcgtctttgggcgaagcg
Construction of {ihfB!} Biobrick Part K112219
PCR mea013/mea014 on MG1655 (316 bp, EcoRI/BamHI/DpnI)
Sub into pBca1256 (EcoRI/BamHI, 2472+910, L)
Product is pBca1256-K112219
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mea013 Forward Biobricking of {ihfB!} ccataGAATTCatgAGATCTatgaccaagtcagaattgatagaaagacttgccaccc
mea014 Reverse Biobricking of {ihfB!} cgttaGGATCCttaaccgtaaatattggcgcgatcg
Construction of {<ihfB!} Biobrick Part K112220
PCR mea015/mea014 on MG1655 (313 bp, EcoRI/BamHI/DpnI)
Sub into pBca1256 (EcoRI/BamHI, 2472+910, L)
Product is pBca1256-K112220
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mea015 Forward Biobricking of {<ihfB!} ccataGAATTCatgAGATCTaccaagtcagaattgatagaaagacttgccaccc
mea014 Reverse Biobricking of {ihfB!} cgttaGGATCCttaaccgtaaatattggcgcgatcg
Construction of {ihfB>} Biobrick Part K112221
PCR mea013/mea016 on MG1655 (313 bp, EcoRI/BamHI/DpnI)
Sub into pBca1256 (EcoRI/BamHI, 2472+910, L)
Product is pBca1256-K112221
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mea013 Forward Biobricking of {ihfB!} ccataGAATTCatgAGATCTatgaccaagtcagaattgatagaaagacttgccaccc
mea016 Reverse Biobricking of {ihfB>} cgttaGGATCCaccgtaaatattggcgcgatc
Construction of {<ihfB>} Biobrick Part K112222
PCR mea015/mea016 on MG1655 (310 bp, EcoRI/BamHI/DpnI)
Sub into pBca1256 (EcoRI/BamHI, 2472+910, L)
Product is pBca1256-K112222
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mea015 Forward Biobricking of {<ihfB!} ccataGAATTCatgAGATCTaccaagtcagaattgatagaaagacttgccaccc
mea016 Reverse Biobricking of {ihfB>} cgttaGGATCCaccgtaaatattggcgcgatc
Construction of {a~ihfB!} Biobrick Part K112223
PCR mea023/mea014 on MG1655 (320 bp, EcoRI/BamHI/DpnI)
Sub into pBca1256 (EcoRI/BamHI, 2472+910, L)
Product is pBca1256-K112223
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mea023 Forward Biobricking of {a~ihfB!}ccataGAATTCatgAGATCTaatcatgaccaagtcagaattgatag
mea014 Reverse Biobricking of {ihfB!} cgttaGGATCCttaaccgtaaatattggcgcgatcg
Construction of {rbs.ihfB!} Biobrick Part K112224
PCR mea024/mea014 on MG1655 (337 bp, EcoRI/BamHI/DpnI)
Sub into pBca1256 (EcoRI/BamHI, 2472+910, L)
Product is pBca1256-K112224
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mea024 Forward Biobricking of {rbs.ihfB!}ccataGAATTCatgAGATCTctttaaggaaccggaggaatcatg
mea014 Reverse Biobricking of {ihfB!} cgttaGGATCCttaaccgtaaatattggcgcgatcg
Construction of {ompT>} Biobrick Part K112225
Wobble PCR mea025/mea026 (97 bp, EcoRI/BamHI/DpnI)
Sub into pBca1256 (EcoRI/BamHI, 2472+910, L)
Product is pBca1256-K112225
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mea025 Forward Biobricking of {ompT>} cgataGAATTCatgAGATCTatgcgggcgaaactcctaggaatagtcctgacaacccctatcg
mea026 Reverse Biobricking of {ompT>} cgttaGGATCCcgtcgacgcaaaagagctgatcgcgataggggttgtcaggactattc
Construction of {a~ompT} Biobrick Part K112226
Wobble PCR mea027/mea026 (101 bp, EcoRI/BamHI/DpnI)
Sub into pBca1256 (EcoRI/BamHI, 2472+910, L)
Product is pBca1256-K112226
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mea027 Forward Biobricking of {a~ompT} cgataGAATTCatgAGATCTacatatgcgggcgaaactcctaggaatagtcctgacaacccctatc
mea026 Reverse Biobricking of {ompT>} cgttaGGATCCcgtcgacgcaaaagagctgatcgcgataggggttgtcaggactattc
Construction of {rbs.ompT} Biobrick Part K112227
Wobble PCR mea028/mea026 (114 bp, EcoRI/BamHI/DpnI)
Sub into pBca1256 (EcoRI/BamHI, 2472+910, L)
Product is pBca1256-K112227
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mea028 Forward Biobricking of {rbs.ompT}cgataGAATTCatgAGATCTaagaaggagatatacatatgcgggcgaaactcctaggaatagtcctgacaacccc
mea026 Reverse Biobricking of {ompT>} cgttaGGATCCcgtcgacgcaaaagagctgatcgcgataggggttgtcaggactattc
Construction of {pelB>} Biobrick Part K112228
Wobble PCR mea029/mea030 (97 bp, EcoRI/BamHI/DpnI)
Sub into pBca1256 (EcoRI/BamHI, 2472+910, L)
Product is pBca1256-K112228
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mea029 Forward Biobricking of {pelB>} cgataGAATTCatgAGATCTatgaaatacctgctgccgaccgctgctgctggtctgctgctcc
mea030 Reverse Biobricking of {pelB>} cgttaGGATCCggccatcgccggctgggcagcgaggagcagcagaccagcagcagcgg
Construction of {a~pelB} Biobrick Part K112229
Wobble PCR mea031/mea030 (101 bp, EcoRI/BamHI/DpnI)
Sub into pBca1256 (EcoRI/BamHI, 2472+910, L)
Product is pBca1256-K112229
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mea031 Forward Biobricking of {a~pelB} cgataGAATTCatgAGATCTacatatgaaatacctgctgccgaccgctgctgctggtctgctg
mea030 Reverse Biobricking of {pelB>} cgttaGGATCCggccatcgccggctgggcagcgaggagcagcagaccagcagcagcgg
Construction of {rbs.pelB} Biobrick Part K112230
Wobble PCR mea032/mea030 (116 bp, EcoRI/BamHI/DpnI)
Sub into pBca1256 (EcoRI/BamHI, 2472+910, L)
Product is pBca1256-K112230
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mea032 Forward Biobricking of {rbs.pelB} cgataGAATTCatgAGATCTttaagaaggagatatacatatgaaatacctgctgccgaccgctgctgctggtctgctg
mea030 Reverse Biobricking of {pelB>} cgttaGGATCCggccatcgccggctgggcagcgaggagcagcagaccagcagcagcgg
Construction of {rbs.xis.int!} Biobrick Part K112234
PCR mea018/mea006 on pAH57 (1317 bp, EcoRI/BamHI/DpnI)
Sub into pBca1256 (EcoRI/BamHI, 2472+910, L)
Product is pBca1256-K112234
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mea018 Forward Biobricking of {rbs.xis!}ccagtGAATTCatgAGATCTtaattgcggagactttgcgatg
mea006 Reverse Biobricking of {int!} cgttaGGATCCttatttgatttcaattttgtcccactccc
