EPF-Lausanne/31 July 2008

From 2008.igem.org

(Difference between revisions)
Line 1: Line 1:
===Ligation of parts===
===Ligation of parts===
-
We digested a few parts yesterday and we ligated them this morning
+
We digested a few parts yesterday and we ran them through a gel. It turns out that some of them are not correct and were badly digested, so we cannot do any ligation, and we have to run the digestions again.
===2 Step PCR===
===2 Step PCR===

Revision as of 08:36, 4 August 2008

Ligation of parts

We digested a few parts yesterday and we ran them through a gel. It turns out that some of them are not correct and were badly digested, so we cannot do any ligation, and we have to run the digestions again.

2 Step PCR

The 2nd step went well. All 3 worked. We will now be able to use these DNA strands to make our transcription fractors and test on the chips to see where they actualy bind.
We have to determine the consensus binding region for each to have a starting point.

Cell culture

We did again a culture from the igem stock the headquarters send us. We cultured the following biobricks : I14033, I1466, R0071, B0034, B0015, P0140, R0040, E1010, F1610.