Team:ESBS-Strasbourg/Transformation Heat shock

From 2008.igem.org

(Difference between revisions)
(New page: * ligation or 10 ng plasmid * add 50-100 µL of competent bacteria * take on ice for 30 min * heat shock at 42 °C for 2 min * add 300 µL LB and incubated at 37°C for 45 min * plate)
 
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Protocol is a mixture of the IGEM standard protocol and the protocol used in the local lab<br>
* ligation or 10 ng plasmid
* ligation or 10 ng plasmid
* add 50-100 µL of competent bacteria
* add 50-100 µL of competent bacteria
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* add 300 µL LB and incubated at 37°C for 45 min
* add 300 µL LB and incubated at 37°C for 45 min
* plate
* plate
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<br>
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back to [[Team:ESBS-Strasbourg/Protocols|Protocols]]

Latest revision as of 08:29, 5 August 2008

Protocol is a mixture of the IGEM standard protocol and the protocol used in the local lab

  • ligation or 10 ng plasmid
  • add 50-100 µL of competent bacteria
  • take on ice for 30 min
  • heat shock at 42 °C for 2 min
  • add 300 µL LB and incubated at 37°C for 45 min
  • plate


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