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Team:University of Ottawa/31 July 2008
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| + | =='''Today in the Lab'''== | ||
| + | |||
| + | =='''Chris'''== | ||
| + | '''Microwave Digestion Experiment''' | ||
| + | :<li> Following protocol outlined online, tested a microwave protocol to hasten digestion period. | ||
| + | :<li> Digested DQ232597 with HindIII. Ran five samples: 5 seconds in micro, 10 seconds, 15 seconds, 10 seconds in and 2 minutes out (four times), water control, water control 10 seconds in microwave and 2 minutes out, 1 hr traditional digest. | ||
| + | :<li> Denatured HindIII at 80 C for 20 minutes, then ran products on a gel. | ||
| + | :<li> Results promising; however, more experimentation needed to confirm validity of this protocol | ||
Revision as of 20:15, 5 August 2008
Today in the Lab
Chris
Microwave Digestion Experiment
- Following protocol outlined online, tested a microwave protocol to hasten digestion period.
- Digested DQ232597 with HindIII. Ran five samples: 5 seconds in micro, 10 seconds, 15 seconds, 10 seconds in and 2 minutes out (four times), water control, water control 10 seconds in microwave and 2 minutes out, 1 hr traditional digest.
- Denatured HindIII at 80 C for 20 minutes, then ran products on a gel.
- Results promising; however, more experimentation needed to confirm validity of this protocol
