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Minnesota/5 August 2008
From 2008.igem.org
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|'''4. Run a gel:''' Using 1% agarose gel, check for correct amount/type of DNA bands. | |'''4. Run a gel:''' Using 1% agarose gel, check for correct amount/type of DNA bands. | ||
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| - | |'''5. Sequencing Rxns:''' Send in sequences. Follow the mixture below, which was sent in today: | + | |'''5. Gel purification: The terminator band was cut out of the electrophoresis gel and purified out using QIAGEN's QuickSpin gel purification protocol. DNA used in ligation below. |
| + | |- | ||
| + | |'''6. Sequencing Rxns:''' Send in sequences. Follow the mixture below, which was sent in today: | ||
Revision as of 20:47, 6 August 2008
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| Go to Previous Day (August 4) | Go to Next Day (August 6) |
| 1. Plasmid Prep: Plasmid prep from the 2mL cultures made from picked colonies of (a) TetR/p22mnt + RFP + Term, (b) Lac/LAMBDA + GFP 1, (c) Lac/LAMBDA + GFP 2, (d) Tetpro + LAMBDAcI + term. | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| 2. Spectrophotometry: Spec'ed the plasmid prep products to check for concentration of DNA. | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| 3. Digest Rxn: Run a digest reaction on the plasmid prep products. Follow the chart below:
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| 4. Run a gel: Using 1% agarose gel, check for correct amount/type of DNA bands. | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| 5. Gel purification: The terminator band was cut out of the electrophoresis gel and purified out using QIAGEN's QuickSpin gel purification protocol. DNA used in ligation below. | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| 6. Sequencing Rxns: Send in sequences. Follow the mixture below, which was sent in today:
|
