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EPF-Lausanne/31 July 2008
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===Ligation of parts=== | ===Ligation of parts=== | ||
| - | We digested a few parts yesterday and we ran them through a gel. It turns out that some of them are not correct and were badly digested, so we cannot do any ligation, and we have to run the digestions again. | + | We digested a few parts yesterday and we ran them through a gel. It turns out that some of them are not correct and were badly digested, so we cannot do any ligation, and we have to run the digestions again, and even grow the DNA again. |
===2 Step PCR=== | ===2 Step PCR=== | ||
Latest revision as of 12:12, 7 August 2008
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Ligation of parts
We digested a few parts yesterday and we ran them through a gel. It turns out that some of them are not correct and were badly digested, so we cannot do any ligation, and we have to run the digestions again, and even grow the DNA again.
2 Step PCR
The 2nd step went well. All 3 worked. We will now be able to use these DNA strands to make our transcription fractors and test on the chips to see where they actualy bind.
We have to determine the consensus binding region for each to have a starting point.
Cell culture
We did again a culture from the igem stock the headquarters send us. We cultured the following biobricks : I14033, I1466, R0071, B0034, B0015, P0140, R0040, E1010, F1610.
