Team:KULeuven/7 August 2008
From 2008.igem.org
(Difference between revisions)
(→Wet Lab) |
(→Dry Lab) |
||
Line 11: | Line 11: | ||
=== Dry Lab === | === Dry Lab === | ||
+ | |||
+ | Cell death contains an error: P1010 is a part which '''constitutively''' produces ccdB. It already contains a promoter and a RBS, see the [http://partsregistry.org/Part:BBa_P1010 parts page] and the [http://partsregistry.org/cgi/partsdb/part_info.cgi?part_name=BBa%20P1010 Hard Information]. | ||
+ | Primers will have to be made to create a full-blown biobrick with just the ccd locus. | ||
== Modeling == | == Modeling == |
Revision as of 14:24, 7 August 2008
<< return to notebook | return to homepage >> | ||
< previous friday | ← yesterday | tomorrow → | next monday > |
Contents |
Lab Work
Wet Lab
- F1610 wasn't cut with XbaI. We are doing this again. The result will follow tomorrow.
- The primers finally arrived and we are doing a PCR on the ligated parts.
- We'll do some ligations today: C0012 + B0015, R0062+B0032, R0084+J23022.
Dry Lab
Cell death contains an error: P1010 is a part which constitutively produces ccdB. It already contains a promoter and a RBS, see the [http://partsregistry.org/Part:BBa_P1010 parts page] and the [http://partsregistry.org/cgi/partsdb/part_info.cgi?part_name=BBa%20P1010 Hard Information]. Primers will have to be made to create a full-blown biobrick with just the ccd locus.