Team:The University of Alberta/7 August 2008

From 2008.igem.org

(Difference between revisions)
(New page: '''Saima''' *PCRed PCAM2201 using Cam35s Forward and Reverse Primers. **Digested the PCR product with Xba1/Pst.Ran them on 1%gel. Gel purified the bands. ***Ligated the gel purified produc...)
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'''Saima'''
'''Saima'''
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*PCRed PCAM2201 using Cam35s Forward and Reverse Primers.
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*PCRed PCAM2201 using Cam35s Forward and Reverse Primers. (Protocol to be followed is given in the Masterbook)
**Digested the PCR product with Xba1/Pst.Ran them on 1%gel. Gel purified the bands.
**Digested the PCR product with Xba1/Pst.Ran them on 1%gel. Gel purified the bands.
***Ligated the gel purified product with J61003.
***Ligated the gel purified product with J61003.

Revision as of 17:20, 7 August 2008

Saima

  • PCRed PCAM2201 using Cam35s Forward and Reverse Primers. (Protocol to be followed is given in the Masterbook)
    • Digested the PCR product with Xba1/Pst.Ran them on 1%gel. Gel purified the bands.
      • Ligated the gel purified product with J61003.
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