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Wisconsin: Lignin Project/12 June 2008
From 2008.igem.org
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|'''Team Sorbitol''' | |'''Team Sorbitol''' | ||
| - | We designed and | + | We designed and ordered primers for cloning ''srlD'' and the ''srl''operon out of E. coli MG1655. |
These primers will also add an xba1 site as well as HindIII site to each end of the gene and operon so they can easily be cloned into a plasmid vector. | These primers will also add an xba1 site as well as HindIII site to each end of the gene and operon so they can easily be cloned into a plasmid vector. | ||
| - | Note: on the ''srlD'' primer we added a Shine-Delgarno sequence so that the gene can be expressed without the operon. | + | '''Note:''' on the ''srlD'' primer we added a Shine-Delgarno sequence so that the gene can be expressed without the operon. |
We also chose pBAD30 and pBAD18 as our starting (and storage) plasmid vectors to clone into. | We also chose pBAD30 and pBAD18 as our starting (and storage) plasmid vectors to clone into. | ||
Latest revision as of 15:11, 8 August 2008
| Team Sorbitol
We designed and ordered primers for cloning srlD and the srloperon out of E. coli MG1655. These primers will also add an xba1 site as well as HindIII site to each end of the gene and operon so they can easily be cloned into a plasmid vector. Note: on the srlD primer we added a Shine-Delgarno sequence so that the gene can be expressed without the operon. We also chose pBAD30 and pBAD18 as our starting (and storage) plasmid vectors to clone into. They are both arabanose inducible and low and high copy number (respectively) |
