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Wisconsin: Lignin Project/17 June 2008
From 2008.igem.org
(Difference between revisions)
| Line 8: | Line 8: | ||
Harvested the chromosomal DNA from the overnight MG1655 cultures by boiling the cells. | Harvested the chromosomal DNA from the overnight MG1655 cultures by boiling the cells. | ||
| - | + | Used this DNA as a template for 2 PCR reactions. | |
| - | Used this DNA as a template for 2 PCR reactions | + | |
| - | + | ||
One reaction was to clone out ''srlD'' the other was to clone the ''srl'' operon. | One reaction was to clone out ''srlD'' the other was to clone the ''srl'' operon. | ||
| - | |||
However when run on a gel no bands were found. | However when run on a gel no bands were found. | ||
Revision as of 15:15, 8 August 2008
| Team Sorbitol:
Harvested the chromosomal DNA from the overnight MG1655 cultures by boiling the cells. Used this DNA as a template for 2 PCR reactions. One reaction was to clone out srlD the other was to clone the srl operon. However when run on a gel no bands were found. Team Fungus: |
