Wisconsin: Lignin Project/17 June 2008

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|'''Team Sorbitol:'''
|'''Team Sorbitol:'''
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Harvested the chromosomal DNA from the overnight MG1655 cultures by boiling the cells.
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Harvested the chromosomal DNA from the overnight MG1655 cultures by boiling the cells.<br>
-
 
+
Used this DNA as a template for 2 PCR reactions.<br>
-
Used this DNA as a template for 2 PCR reactions
+
One reaction was to clone out ''srlD'' the other was to clone the ''srl'' operon.<br>
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+
However when run on a gel no bands were found.<br>
-
One reaction was to clone out ''srlD'' the other was to clone the ''srl'' operon.
+
-
 
+
-
However when run on a gel no bands were found.
+
'''Team Fungus:''' <br>
'''Team Fungus:''' <br>

Latest revision as of 15:15, 8 August 2008

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Team Sorbitol:

Harvested the chromosomal DNA from the overnight MG1655 cultures by boiling the cells.
Used this DNA as a template for 2 PCR reactions.
One reaction was to clone out srlD the other was to clone the srl operon.
However when run on a gel no bands were found.

Team Fungus:
Received primers
Diluted primers
Ran TAQ PCR reaction with Carbon-limited cDNA
Ran PCR product on 1% agrose gel; only ladder visible