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Wisconsin: Lignin Project/18 June 2008
From 2008.igem.org
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(New page: '''Team Fungus:''' <br> Ran Carbon-limited and Nitrogen-limited cDNA on 1% agrose gel<br> -Only ladder showed<br> Analysis: cDNA is single stranded and ethidium bromide dye does bind.) |
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| + | |'''Team Sorbitol:''' | ||
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| + | Repeated the PCRs from yesterday, but harvested the chromosomal DNA using proteinase K instead of boiling.<br> | ||
| + | When the PCR product was run on a gel we found a band around 700bp (the correct size of ''srlD'')<br> | ||
| + | Also attended the Wisconsin iGEM team meeting.<br> | ||
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'''Team Fungus:''' <br> | '''Team Fungus:''' <br> | ||
Ran Carbon-limited and Nitrogen-limited cDNA on 1% agrose gel<br> | Ran Carbon-limited and Nitrogen-limited cDNA on 1% agrose gel<br> | ||
-Only ladder showed<br> | -Only ladder showed<br> | ||
| - | Analysis: cDNA is single stranded and ethidium bromide dye does bind. | + | -Analysis: cDNA is single stranded and ethidium bromide dye does not bind. <br> |
| + | iGEM team meeting <br> | ||
| + | |} | ||
Latest revision as of 15:17, 8 August 2008
| Team Sorbitol:
Repeated the PCRs from yesterday, but harvested the chromosomal DNA using proteinase K instead of boiling. Team Fungus: |
