Wisconsin: Lignin Project/18 June 2008

From 2008.igem.org

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|'''Team Sorbitol:'''
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Repeated the PCRs from yesterday, but harvested the chromosomal DNA using proteinase K instead of boiling.<br>
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When the PCR product was run on a gel we found a band around 700bp (the correct size of ''srlD'')<br>
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Also attended the Wisconsin iGEM team meeting.<br>
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'''Team Fungus:''' <br>
'''Team Fungus:''' <br>
Ran Carbon-limited and Nitrogen-limited cDNA on 1% agrose gel<br>
Ran Carbon-limited and Nitrogen-limited cDNA on 1% agrose gel<br>
-Only ladder showed<br>
-Only ladder showed<br>
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Analysis: cDNA is single stranded and ethidium bromide dye does not bind.
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-Analysis: cDNA is single stranded and ethidium bromide dye does not bind. <br>
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iGEM team meeting <br>
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Latest revision as of 15:17, 8 August 2008

Igemwibanner.gif
Team Sorbitol:

Repeated the PCRs from yesterday, but harvested the chromosomal DNA using proteinase K instead of boiling.
When the PCR product was run on a gel we found a band around 700bp (the correct size of srlD)
Also attended the Wisconsin iGEM team meeting.

Team Fungus:
Ran Carbon-limited and Nitrogen-limited cDNA on 1% agrose gel
-Only ladder showed
-Analysis: cDNA is single stranded and ethidium bromide dye does not bind.
iGEM team meeting