Wisconsin: Lignin Project/25 June 2008

From 2008.igem.org

(Difference between revisions)

Latest revision as of 15:25, 8 August 2008

Team Sorbitol:

Attended the Wisconsin iGEM team meeting
Re-evaluated our PCR reactions and primers
Decided to alter the temperatures and times of several steps.
Also we redesigned the primers used to clone out the srl operon.

Team Fungus:
Digested PCR product and pET28a vector
Ran 1% agrose gel of digestion and performed gel purification
-digested and undigested PCR product as well as digested vector showed correctly sized bands
-undigested vector showed up at about 3kb when compared to supercoil ladder and at 4kb when compared to linear 1kb ladder
Ran the rest of digestion reaction out on another 1% agrose gel and purified using QIAquick gel extraction kit

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+<div style="background: #000; padding: 10px;">
+<div style="width:800px; height:147px; border:0px; margin:0px;">[[Image:Igemwibanner.gif]]</div>
+{|align="justify" style="color:#aada84;background-color:#000;" width="800 px"
+|'''Team Sorbitol:'''<br>
+Attended the Wisconsin iGEM team meeting<br>
+Re-evaluated our PCR reactions and primers<br>
+Decided to alter the temperatures and times of several steps.<br>
+Also we redesigned the primers used to clone out the ''srl'' operon.<br>
 '''Team Fungus:''' <br>
-Ran digestion of cDNA into pET28a vector<br>
+Digested PCR product and pET28a vector<br>
-Ran gel of digestion and performed gel purification<br>
+Ran 1% agrose gel of digestion and performed gel purification<br>
+-digested and undigested PCR product as well as digested vector showed correctly sized bands <br>
+-undigested vector showed up at about 3kb when compared to supercoil ladder and at 4kb when compared to linear 1kb ladder <br>
+Ran the rest of digestion reaction out on another 1% agrose gel and purified using QIAquick gel extraction kit
+|}