Wisconsin: Lignin Project/1 July 2008

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'''Team Sorbitol:'''<br>
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|'''Team Sorbitol:'''<br>
The overnight PCR reaction failed.<br>
The overnight PCR reaction failed.<br>
Used the purified PCR product from yesterday to set up a digestion (along with pBAD30) as described before.<br>
Used the purified PCR product from yesterday to set up a digestion (along with pBAD30) as described before.<br>

Revision as of 15:30, 8 August 2008

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Team Sorbitol:

The overnight PCR reaction failed.
Used the purified PCR product from yesterday to set up a digestion (along with pBAD30) as described before.
The digestion was verified on a gel and extracted using the Qiagen kit.
This time we obtained enough insert to do a ligation but not enough vector.

To solve this we grew 40mL of DH5a with pBAD30 overnight at 30C
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