Template:Team:UC Berkeley/Notebook/SC construction

From 2008.igem.org

(Difference between revisions)

Latest revision as of 20:23, 8 August 2008

1 pBca1256-K112600
2 pBca1256-K112601
3 pBca1256-K112602
4 pBca1256-K112603
5 pBca1256-K112604
6 pBjh1601AC-K112403
7 pBjh1601AC-K112404
8 pBjh1601KC-K112605
9 pBjh1601CK-K112606
10 pBjh1601KC-K112607
11 pBjh1601KC-K112608
12 pBjh1601CA-K112609
13 pBca1256-K112610
14 pBca1256-K112611
15 pBca1256-K112612
16 pBca1256-K112613
17 pBca1256-K112614
18 pBca1256-K112615
19 pBca1102-Bca9194 Assembly part

pBca1256-K112600

Construction of {<AP>} basic part K112600

Wobble PCR SC001/SC002            (66 bp, EcoRI/BamHI)
Sub into pBca1256                 (EcoRI/BamHI, 2472+ 910)
Product is pBca1256-K112600
----------------------------------------------------
SC001F    Forward Biobricking of <AP-tag>
cgataGAATTCatgAGATCTggcctgaacgatatttttgaagcgcag
SC002R    Reverse Biobricking of <AP-tag>
ccagtGGATCCttcatgccattcaattttctgcgcttcaaaaatatcg

pBca1256-K112601

Construction of {<AP!} basic part K112601

Wobble PCR SC001/SC003            (69 bp, EcoRI/BamHI)
Sub into pBca1256                 (EcoRI/BamHI, 2472+ 910)
Product is pBca1256-K112601
--------------------------------------------------
SC001    Forward Biobricking of <AP-tag!
cgataGAATTCatgAGATCTggcctgaacgatatttttgaagcgcag
SC003    Reverse Biobricking of <AP-tag!
ccagtGGATCCttattcatgccattcaattttctgcgcttcaaaaatatcg

pBca1256-K112602

Construction of {<FLAG>} basic part K112602

Wobble PCR SC004/SC005            (45 bp, EcoRI/BamHI)
Sub into pBca1256                 (EcoRI/BamHI, 2472+ 910)
Product is pBca1256-K112602
---------------------------------------------------
SC004     Forward Biobricking of <FLAG-tag>
cgataGAATTCatgAGATCTgactacaaggatgacgacg
SC005     Reverse Biobricking of <FLAG-tag>
ccagtGGATCCcttgtcgtcgtcatccttgtagtc

pBca1256-K112603

Construction of {<FLAG!} basic part K112603

Wobble PCR SC004/SC006            (48 bp, EcoRI/BamHI)
Sub into pBca1256                 (EcoRI/BamHI, 2472+ 910)
Product is pBca1256-K112603
-----------------------------------------------------
SC004    Forward Biobricking of <FLAG-tag!
cgataGAATTCatgAGATCTgactacaaggatgacgacg
SC006    Reverse Biobricking of <FLAG-tag!
ccagtGGATCCttacttgtcgtcgtcatccttgtagtc

pBca1256-K112604

Construction of {rbs_barstar>} basic part K112604

PCR mea035/sc007 on Bacillus amyloliquefaciens gen.            (320 bp, EcoRI/BamHI)
Sub into pBca1256                                              (EcoRI/BamHI, 2472+910, L)
Product is pBca1256-K112604
-------------------------------
mea035    Fwd biobricking of {rbs_barstar>} 
cgataGAATTCatgAGATCTcataagaaaggagccgcacatg                              
sc007     Reverse biobricking of {rbs_barstar>}                     (Not needed...)
cgttaGGATCCagaaagtatgatggtgatgtcgc

pBjh1601AC-K112403

Construction of {PhoA>} basic part K112403
          
CR cb10007/cb10008 on MG1655            (95 bp, EcoRI/BamHI)                              
Sub into pBjh1601AC                       (EcoRI/BamHI, 3195+910 L)
Product is pBjh1601AC-K112403                                            
-------------------------------------------------------------
cb10007    Fwd biobricking of {prepro>}
cgataGAATTCatgAGATCTatgaaacaaagcactattgcactggc
cb10008    Reverse biobricking of {prepro>}
cgtagGGATCCgggcttttgtcacaggggtaaac

pBjh1601AC-K112404

Construction of {LamB>} basic part K112404   
           
PCR cb10009/cb10010 on MG1655            (107 bp, EcoRI/BamHI)                                 
Sub into pBjh1601AC                     (EcoRI/BamHI, 3195+910, L)                                               
Product is pBjh1601AC-K112404                                           
-------------------------------------------------------------
cb10009    Fwd biobricking of {prepro>}
cgataGAATTCatgAGATCTatgatgattactctgcgcaaac
cb10010    Reverse biobricking of {prepro>}
cgtagGGATCCcagccattgcctgagcagacattac

pBjh1601KC-K112605

Construction of {a~PhoA>} basic part K112605    
         
PCR sc008/cb10008-phoA-R on MG1655            (100 bp, EcoRI/BamHI)                              
Sub into pBjh1601KC                             (EcoRI/BamHI, 3132+910, L)
Product is pBjh1601KC-K112605
------------------------------------------------------
sc008      Fwd biobricking of {a~prepro>}
cgataGAATTCatgAGATCTtaaaGtgaaacaaagcactattgcactggc
cb10008    Reverse biobricking of {a~prepro>}
cgtagGGATCCgggcttttgtcacaggggtaaac

pBjh1601CK-K112606

Construction of {a~LamB>} basic part K112606 
                
PCR sc009/cb10010-lamB-R on MG1655            (111 bp, EcoRI/BamHI)                               
Sub into pBjh1601CK                             (EcoRI/BamHI, 3134+910, L)
Product is pBjh1601CK-K112606
--------------------------------------------------
sc009      Fwd biobricking of {a~prepro>}
cgataGAATTCatgAGATCTtagaatgatgattactctgcgc
cb10010    Reverse biobricking of {a~prepro>}
cgtagGGATCCcagccattgcctgagcagacattac

pBjh1601KC-K112607

Construction of {rbs_PhoA>} basic part K112607               

PCR sc010/cb10008-PhoA-R on MG1655            (113 bp, EcoRI/BamHI)                         
Sub into pBjh1601KC                             (EcoRI/BamHI, 3132+910, L)
Product is pBjh1601KC-K112607
------------------------------------------------------
sc010      Fwd biobricking of {rbs_prepro>}
cgataGAATTCatgAGATCTgtacatggagaaaataaaAtgaaacaaagcac
cb10008    Reverse biobricking of {rbs_prepro>}
cgtagGGATCCgggcttttgtcacaggggtaaac

pBjh1601KC-K112608

Construction of {rbs_LamB>} basic part K112608   
      
PCR sc011/cb10010-lamB-R on E.coli K12 MG1655            (126 bp, EcoRI/BamHI)             
Sub into pBjh1601KC                                        (EcoRI/BamHI, 3132+910, L)
Product is pBjh1601KC-K112608
------------------------------------------------------
sc011      Fwd biobricking of {rbs_prepro>}
cgataGAATTCatgAGATCTcaatgactcaggagatagaatg
cb10010    Reverse biobricking of {rbs_prepro>}
cgtagGGATCCcagccattgcctgagcagacattac

pBjh1601CA-K112609

Construction of {a~PhoA>} basic part K112609 (K112605 replacement)
         
PCR sc012/cb10008-phoA-R on MG1655            (100 bp, EcoRI/BamHI)                              
Sub into pBjh1601CA                             (EcoRI/BamHI, 2472+910, L)
Product is pBjh1601CA-K112605
------------------------------------------------------
sc012      Fwd biobricking of {a~prepro>}
cgataGAATTCatgAGATCTtaaaAtgaaacaaagcactattgcactggc
cb10008    Reverse biobricking of {a~prepro>}
cgtagGGATCCgggcttttgtcacaggggtaaac

pBca1256-K112610

Construction of {PhoA>} basic part K112610
         
PCR cb10007/sc013 on MG1655                   (94 bp, EcoRI/BamHI)                              
Sub into pBca1256                             (EcoRI/BamHI, 2472+910, L)
Product is pBca1256-K112610
------------------------------------------------------
cb10007    Fwd biobricking of {prepro>}
cgataGAATTCatgAGATCTatgaaacaaagcactattgcactggc
sc013    Reverse biobricking of {prepro>}
ccagtGGATCCggcttttgtcacaggggtaaac

pBca1256-K112611

Construction of {LamB>} basic part K112611 
         
PCR cb10009/sc014 on MG1655                   (107 bp, EcoRI/BamHI)                              
Sub into pBca1256                             (EcoRI/BamHI, 2472+910, L)
Product is pBca1256-K112605
------------------------------------------------------
cb10009    Fwd biobricking of {prepro>}
cgataGAATTCatgAGATCTatgatgattactctgcgcaaac
sc014    Reverse biobricking of {prepro>}
ccagtGGATCCagccattgcctgagcagac

pBca1256-K112612

Construction of {a~PhoA>} basic part K112612 
         
PCR sc012/sc013 on MG1655                     (98 bp, EcoRI/BamHI)                              
Sub into pBca1256                             (EcoRI/BamHI, 2472+910, L)
Product is pBca1256-K112605
------------------------------------------------------
sc012      Fwd biobricking of {a~prepro>}
cgataGAATTCatgAGATCTtaaaAtgaaacaaagcactattgcactggc
sc013    Reverse biobricking of {prepro>}
ccagtGGATCCggcttttgtcacaggggtaaac

pBca1256-K112613

Construction of {a~LamB>} basic part K112613
         
PCR sc009/sc014 on MG1655                     (111 bp, EcoRI/BamHI)                              
Sub into pBca1256                             (EcoRI/BamHI, 2472+910, L)
Product is pBca1256-K112605
------------------------------------------------------
sc009      Fwd biobricking of {a~prepro>}
cgataGAATTCatgAGATCTtagaatgatgattactctgcgc
sc014    Reverse biobricking of {prepro>}
ccagtGGATCCagccattgcctgagcagac

pBca1256-K112614

Construction of {rbs_PhoA>} basic part K112614
         
PCR sc010/sc013 on MG1655                     (112 bp, EcoRI/BamHI)                              
Sub into pBca1256                             (EcoRI/BamHI, 2472+910, L)
Product is pBca1256-K112605
------------------------------------------------------
sc010      Fwd biobricking of {rbs_prepro>}
cgataGAATTCatgAGATCTgtacatggagaaaataaaAtgaaacaaagcac
sc013    Reverse biobricking of {prepro>}
ccagtGGATCCggcttttgtcacaggggtaaac

pBca1256-K112615

Construction of {rbs_LamB>} basic part K112615

PCR sc011/sc014 on MG1655                     (126 bp, EcoRI/BamHI)                              
Sub into pBca1256                             (EcoRI/BamHI, 2472+910, L)
Product is pBca1256-K112605
------------------------------------------------------
sc011      Fwd biobricking of {rbs_prepro>}
cgataGAATTCatgAGATCTcaatgactcaggagatagaatg
sc014    Reverse biobricking of {prepro>}
ccagtGGATCCagccattgcctgagcagac

pBca1102-Bca9194 Assembly part

PCR ca998/mea037 on Bca9194                           (1610 bp, EcoRI/BamHI?)
Sub into {<tag!}.{b1006} composite part (CK vector)   (EcoRI/BamHI,____+__) 
Product is _______________
---------------------------------------------------------
ca998     Fwd biobricking 
gtatcacgaggcagaatttcag
mea037    Reverse biobricking of {<phoA>}
cgttaGGATCCCTTCAGGCCCAGCGCCGCTTTCATG

Sherine Cheung

Back to Berkeley Team Homepage

All Notebooks

@@ Line 1: / Line 1: @@
+<span style="font-family: Century Gothic; font-size: 10pt">
+<div style="text-align: right;"> [[Team:UC_Berkeley/Notebook/Sherine_Cheung|Back to Sherine's Notebook]] </div>
+<div style="text-align: right;"> [[Team:UC_Berkeley/Notebook| To All Notebooks]] </div>
 __TOC__
@@ Line 4: / Line 7: @@
 == pBca1256-K112600 ==
 <pre>
-Construction of <AP-tag> basic part K112600
+Construction of {<AP>} basic part K112600
 Wobble PCR SC001/SC002            (66 bp, EcoRI/BamHI)
-Sub into pBca1256                 (EcoRI/BamHI, 2227+ 910)
+Sub into pBca1256                 (EcoRI/BamHI, 2472+ 910)
 Product is pBca1256-K112600
 ----------------------------------------------------
@@ Line 18: / Line 21: @@
 == pBca1256-K112601 ==
 <pre>
-Construction of <AP-tag! basic part K112601
+Construction of {<AP!} basic part K112601
 Wobble PCR SC001/SC003            (69 bp, EcoRI/BamHI)
-Sub into pBca1256                 (EcoRI/BamHI, 2227+ 910)
+Sub into pBca1256                 (EcoRI/BamHI, 2472+ 910)
 Product is pBca1256-K112601
 --------------------------------------------------
@@ Line 32: / Line 35: @@
 == pBca1256-K112602 ==
 <pre>
-Construction of <FLAG-tag> basic part K112602
+Construction of {<FLAG>} basic part K112602
 Wobble PCR SC004/SC005            (45 bp, EcoRI/BamHI)
-Sub into pBca1256                 (EcoRI/BamHI, 2227+ 910)
+Sub into pBca1256                 (EcoRI/BamHI, 2472+ 910)
 Product is pBca1256-K112602
 ---------------------------------------------------
@@ Line 46: / Line 49: @@
 == pBca1256-K112603 ==
 <pre>
-Construction of <FLAG-tag! basic part K112603
+Construction of {<FLAG!} basic part K112603
 Wobble PCR SC004/SC006            (48 bp, EcoRI/BamHI)
-Sub into pBca1256                 (EcoRI/BamHI, 2227+ 910)
+Sub into pBca1256                 (EcoRI/BamHI, 2472+ 910)
 Product is pBca1256-K112603
 -----------------------------------------------------
@@ Line 72: / Line 75: @@
 </pre>
-== pBca1256-K112403 ==
+== pBjh1601AC-K112403 ==
 <pre>
-Construction of {prepro>} of PhoA basic part K112403
+Construction of {PhoA>} basic part K112403
 CR cb10007/cb10008 on MG1655            (95 bp, EcoRI/BamHI)
-Sub into pBca1256                       (EcoRI/BamHI, 2472+910, L)
+Sub into pBjh1601AC                       (EcoRI/BamHI, 3195+910 L)
-Product is pBca1256-K112403
+Product is pBjh1601AC-K112403
 -------------------------------------------------------------
 cb10007    Fwd biobricking of {prepro>}
@@ Line 86: / Line 89: @@
 </pre>
-== pBca1256-K112404  ==
+== pBjh1601AC-K112404  ==
 <pre>
-Construction of {prepro>} of LamB basic part K112404
+Construction of {LamB>} basic part K112404
 PCR cb10009/cb10010 on MG1655            (107 bp, EcoRI/BamHI)
-Sub into pBca1256                        (EcoRI/BamHI, 2472+910, L)
+Sub into pBjh1601AC                     (EcoRI/BamHI, 3195+910, L)
-Product is pBca1256-K112404
+Product is pBjh1601AC-K112404
 -------------------------------------------------------------
 cb10009    Fwd biobricking of {prepro>}
@@ Line 100: / Line 103: @@
 </pre>
-== pBca1256-K112605 (needs to be redone -- replace with Atg)==
+== pBjh1601KC-K112605 ==
 <pre>
-Construction of {a~prepro>} of PhoA basic part K112605
+Construction of {a~PhoA>} basic part K112605
 PCR sc008/cb10008-phoA-R on MG1655            (100 bp, EcoRI/BamHI)
-Sub into pBca1256                             (EcoRI/BamHI, 2472+910, L)
+Sub into pBjh1601KC                             (EcoRI/BamHI, 3132+910, L)
-Product is pBca1256-K112605
+Product is pBjh1601KC-K112605
 ------------------------------------------------------
 sc008      Fwd biobricking of {a~prepro>}
@@ Line 114: / Line 117: @@
 </pre>
-== pBca1256-K112606 ==
+== pBjh1601CK-K112606 ==
 <pre>
-Construction of {a~prepro>} of LamB basic part K112606
+Construction of {a~LamB>} basic part K112606
 PCR sc009/cb10010-lamB-R on MG1655            (111 bp, EcoRI/BamHI)
-Sub into pBca1256                             (EcoRI/BamHI, 2472+910, L)
+Sub into pBjh1601CK                             (EcoRI/BamHI, 3134+910, L)
-Product is pBca1256- K112606
+Product is pBjh1601CK-K112606
 --------------------------------------------------
 sc009      Fwd biobricking of {a~prepro>}
@@ Line 128: / Line 131: @@
 </pre>
-== pBca1256-K112607  ==
+== pBjh1601KC-K112607  ==
 <pre>
-Construction of {rbs_prepro>} of PhoA basic part K112607
+Construction of {rbs_PhoA>} basic part K112607
 PCR sc010/cb10008-PhoA-R on MG1655            (113 bp, EcoRI/BamHI)
-Sub into pBca1256                             (EcoRI/BamHI, 2472+910, L)
+Sub into pBjh1601KC                             (EcoRI/BamHI, 3132+910, L)
-Product is pBca1256-K112607
+Product is pBjh1601KC-K112607
 ------------------------------------------------------
 sc010      Fwd biobricking of {rbs_prepro>}
@@ Line 142: / Line 145: @@
 </pre>
-== pBca1256-K112608 ==
+== pBjh1601KC-K112608 ==
 <pre>
-Construction of {rbs_prepro>} of LamB basic part  K112608
+Construction of {rbs_LamB>} basic part K112608
 PCR sc011/cb10010-lamB-R on E.coli K12 MG1655            (126 bp, EcoRI/BamHI)
-Sub into pBca1256                                        (EcoRI/BamHI, 2472+910, L)
+Sub into pBjh1601KC                                        (EcoRI/BamHI, 3132+910, L)
-Product is pBca1256-K112608
+Product is pBjh1601KC-K112608
 ------------------------------------------------------
 sc011      Fwd biobricking of {rbs_prepro>}
@@ Line 156: / Line 159: @@
 </pre>
+== pBjh1601CA-K112609 ==
+<pre>
+Construction of {a~PhoA>} basic part K112609 (K112605 replacement)
+PCR sc012/cb10008-phoA-R on MG1655            (100 bp, EcoRI/BamHI)
+Sub into pBjh1601CA                             (EcoRI/BamHI, 2472+910, L)
+Product is pBjh1601CA-K112605
+------------------------------------------------------
+sc012      Fwd biobricking of {a~prepro>}
+cgataGAATTCatgAGATCTtaaaAtgaaacaaagcactattgcactggc
+cb10008    Reverse biobricking of {a~prepro>}
+cgtagGGATCCgggcttttgtcacaggggtaaac
+</pre>
+---------------------------------------------------------------------------------------------------------------
+== pBca1256-K112610 ==
+<pre>
+Construction of {PhoA>} basic part K112610
+PCR cb10007/sc013 on MG1655                   (94 bp, EcoRI/BamHI)
+Sub into pBca1256                             (EcoRI/BamHI, 2472+910, L)
+Product is pBca1256-K112610
+------------------------------------------------------
+cb10007    Fwd biobricking of {prepro>}
+cgataGAATTCatgAGATCTatgaaacaaagcactattgcactggc
+sc013    Reverse biobricking of {prepro>}
+ccagtGGATCCggcttttgtcacaggggtaaac
+</pre>
+== pBca1256-K112611 ==
+<pre>
+Construction of {LamB>} basic part K112611
+PCR cb10009/sc014 on MG1655                   (107 bp, EcoRI/BamHI)
+Sub into pBca1256                             (EcoRI/BamHI, 2472+910, L)
+Product is pBca1256-K112605
+------------------------------------------------------
+cb10009    Fwd biobricking of {prepro>}
+cgataGAATTCatgAGATCTatgatgattactctgcgcaaac
+sc014    Reverse biobricking of {prepro>}
+ccagtGGATCCagccattgcctgagcagac
+</pre>
+== pBca1256-K112612 ==
+<pre>
+Construction of {a~PhoA>} basic part K112612
+PCR sc012/sc013 on MG1655                     (98 bp, EcoRI/BamHI)
+Sub into pBca1256                             (EcoRI/BamHI, 2472+910, L)
+Product is pBca1256-K112605
+------------------------------------------------------
+sc012      Fwd biobricking of {a~prepro>}
+cgataGAATTCatgAGATCTtaaaAtgaaacaaagcactattgcactggc
+sc013    Reverse biobricking of {prepro>}
+ccagtGGATCCggcttttgtcacaggggtaaac
+</pre>
+== pBca1256-K112613 ==
+<pre>
+Construction of {a~LamB>} basic part K112613
+PCR sc009/sc014 on MG1655                     (111 bp, EcoRI/BamHI)
+Sub into pBca1256                             (EcoRI/BamHI, 2472+910, L)
+Product is pBca1256-K112605
+------------------------------------------------------
+sc009      Fwd biobricking of {a~prepro>}
+cgataGAATTCatgAGATCTtagaatgatgattactctgcgc
+sc014    Reverse biobricking of {prepro>}
+ccagtGGATCCagccattgcctgagcagac
+</pre>
+== pBca1256-K112614 ==
+<pre>
+Construction of {rbs_PhoA>} basic part K112614
+PCR sc010/sc013 on MG1655                     (112 bp, EcoRI/BamHI)
+Sub into pBca1256                             (EcoRI/BamHI, 2472+910, L)
+Product is pBca1256-K112605
+------------------------------------------------------
+sc010      Fwd biobricking of {rbs_prepro>}
+cgataGAATTCatgAGATCTgtacatggagaaaataaaAtgaaacaaagcac
+sc013    Reverse biobricking of {prepro>}
+ccagtGGATCCggcttttgtcacaggggtaaac
+</pre>
+== pBca1256-K112615 ==
+<pre>
+Construction of {rbs_LamB>} basic part K112615
+PCR sc011/sc014 on MG1655                     (126 bp, EcoRI/BamHI)
+Sub into pBca1256                             (EcoRI/BamHI, 2472+910, L)
+Product is pBca1256-K112605
+------------------------------------------------------
+sc011      Fwd biobricking of {rbs_prepro>}
+cgataGAATTCatgAGATCTcaatgactcaggagatagaatg
+sc014    Reverse biobricking of {prepro>}
+ccagtGGATCCagccattgcctgagcagac
+</pre>
+== pBca1102-Bca9194 Assembly part ==
+<pre>
+PCR ca998/mea037 on Bca9194                           (1610 bp, EcoRI/BamHI?)
+Sub into {<tag!}.{b1006} composite part (CK vector)   (EcoRI/BamHI,____+__)
+Product is _______________
+---------------------------------------------------------
+ca998     Fwd biobricking
+gtatcacgaggcagaatttcag
+mea037    Reverse biobricking of {<phoA>}
+cgttaGGATCCCTTCAGGCCCAGCGCCGCTTTCATG
+</pre>
 ----
 <div style="text-align: center;"> [[Team:UC_Berkeley/Notebook/Sherine_Cheung|Sherine Cheung]] </div>
 <div style="text-align: center;"> [[Team:UC_Berkeley|Back to Berkeley Team Homepage]] </div>
+<div style="text-align: center;"> [[Team:UC_Berkeley/Notebook| All Notebooks]] </div>

Template:Team:UC Berkeley/Notebook/SC construction

From 2008.igem.org

Latest revision as of 20:23, 8 August 2008

Contents

pBca1256-K112600

pBca1256-K112601

pBca1256-K112602

pBca1256-K112603

pBca1256-K112604

pBjh1601AC-K112403

pBjh1601AC-K112404

pBjh1601KC-K112605

pBjh1601CK-K112606

pBjh1601KC-K112607

pBjh1601KC-K112608

pBjh1601CA-K112609

pBca1256-K112610

pBca1256-K112611

pBca1256-K112612

pBca1256-K112613

pBca1256-K112614

pBca1256-K112615

pBca1102-Bca9194 Assembly part