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Team:KULeuven/11 August 2008
From 2008.igem.org
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== Modeling == | == Modeling == | ||
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| + | We found a small (but important) mistake in our model: the efficiency of the rbs for the translation of LacI was set to ony 0.05 while all the other efficienties of the rbs that are incorporated in the lock3d were put on 0.167. This makes a serious difference in the final result and not in a good way: the amount of produced LuxI (and following HSL/HSL_LuxR-complex) is reduced a lot! We we're able to slightly repair this new fact by increasing the efficiency of the rbs of translation of LuxI to maximal, but this doesn't completely fix the problem. It only results in the formation of 2 to 3 molecules of ccdB in the cell which might not be lethal enough... | ||
== Wiki == | == Wiki == | ||
Revision as of 11:33, 11 August 2008
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Contents |
Lab Work
Wet Lab
Dry Lab
Modeling
We found a small (but important) mistake in our model: the efficiency of the rbs for the translation of LacI was set to ony 0.05 while all the other efficienties of the rbs that are incorporated in the lock3d were put on 0.167. This makes a serious difference in the final result and not in a good way: the amount of produced LuxI (and following HSL/HSL_LuxR-complex) is reduced a lot! We we're able to slightly repair this new fact by increasing the efficiency of the rbs of translation of LuxI to maximal, but this doesn't completely fix the problem. It only results in the formation of 2 to 3 molecules of ccdB in the cell which might not be lethal enough...
Wiki
Quote of the day
“It is better to be roughly right than precisely wrong” - [J.M. Keynes]
Remarks
Multicroc is gesloten tot 27 aug!
