Wisconsin: Lignin Project/7 July 2008

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We found that each colony contained accurate bands indicating pBAD30 as well as ''srlD''.<br>
We found that each colony contained accurate bands indicating pBAD30 as well as ''srlD''.<br>
Plated these accurate colonies and stored them all in a fridge at 4C as well as grew some colonies overnight.<br>
Plated these accurate colonies and stored them all in a fridge at 4C as well as grew some colonies overnight.<br>
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'''Team Fungus:'''<br>
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Ran PCR using TAQ and only N-limited cDNA.<br>
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Revision as of 16:24, 11 August 2008

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Team Sorbitol:

The 10 colonies were harvested with a Qiagen Miniprep Kit and the DNA was digested with Xba1 and HindIII for 1 hour at 37C.
The digestion was then run on an agarose gel along with controls and undigested vector.
We found that each colony contained accurate bands indicating pBAD30 as well as srlD.
Plated these accurate colonies and stored them all in a fridge at 4C as well as grew some colonies overnight.
Team Fungus:
Ran PCR using TAQ and only N-limited cDNA.

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