Wisconsin: Lignin Project/23 July 2008

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(Difference between revisions)

Revision as of 16:39, 11 August 2008

Team Sorbitol:

Sucessfully cloned out the srl operon and srlD with restriction enzymes for an insertion into pET28a.
Team Fungus:
Minipreped to purify pET28a from overnight culture
The purified vector was put in 2 tubes with spec readings of 112.2 and 130.6 ng/uL respectively.

@@ Line 7: / Line 7: @@
 Sucessfully cloned out the ''srl'' operon and ''srlD'' with restriction enzymes for an insertion into pET28a.<br>
 '''Team Fungus:'''<br>
-Ran overnight cultures of pET28a in LB + KAN (30ug/mL).<br>
+Minipreped to purify pET28a from overnight culture<br>
+The purified vector was put in 2 tubes with spec readings of 112.2 and 130.6 ng/uL respectively. <br>
 |}