Team:Paris/Modeling/f1

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No particular strain.
No particular strain.
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[[Image:f1a.png]]
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[[Image:f2alt.png]]
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In phase of exponential growth, identify the steady state of the Fluorescence/OD, for given values of IPTG introduced. Several approaches are proposed [[Team:Paris/Modeling/estimation#how_to_control_the_concentration_of_the_transcription_factor_.3F|here ]], in order to find the relation between [IPTG] and the expression of ''Plac''.
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To deduce from our measurements the correct hill function, see the [[Team:Paris/Modeling/estimation#how_to_control_the_concentration_of_the_transcription_factor_.3F|different methods]].
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The best one consist in finding in the literature the dissociation constant of the reaction aTc + TetR&#8644; aTc_TetR, in order to determine by calculus the amount of free TetR, function of the expression of a known constitutive promoter before ''tetR'' and of the added aTc. In this way, we just have to study [expr.''Ptet''] = &#131;2(TetR<sub>free</sub>).

Revision as of 15:09, 13 August 2008

No particular strain.

F2alt.png

To deduce from our measurements the correct hill function, see the different methods. The best one consist in finding in the literature the dissociation constant of the reaction aTc + TetR⇄ aTc_TetR, in order to determine by calculus the amount of free TetR, function of the expression of a known constitutive promoter before tetR and of the added aTc. In this way, we just have to study [expr.Ptet] = ƒ2(TetRfree).

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