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Team:Montreal/Notebook/August
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Horiavulpe (Talk | contribs) (New page: == August 2008 == === August 12 === *Seeded the T9002 plasmid in BL21 A1s in the following way: all tubes contain 3mL of medium 1. Control M9+Yeast +dextrose with Amp 2. T9002 colony...) |
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Colony 1 is in two tubes: M9 and LB. If the M9 is bad we'll know because it will have grown in LB. If it doesn't grow in either then something's up. | Colony 1 is in two tubes: M9 and LB. If the M9 is bad we'll know because it will have grown in LB. If it doesn't grow in either then something's up. | ||
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| + | === August 13 === | ||
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| + | *Diluted culture #2 of T9002 in M9/Yeast in three tubes of 11x, 22x and 44x. | ||
Revision as of 20:59, 13 August 2008
August 2008
August 12
- Seeded the T9002 plasmid in BL21 A1s in the following way:
all tubes contain 3mL of medium
1. Control M9+Yeast +dextrose with Amp
2. T9002 colony 1 in LB +Amp
3. T9002 colony 2 in M9/Yeast + dextrose +Amp
4. T9002 colony 1 in M9/Yeast + dextrose +Amp
@ 9:15 PM
Colony 1 is in two tubes: M9 and LB. If the M9 is bad we'll know because it will have grown in LB. If it doesn't grow in either then something's up.
August 13
- Diluted culture #2 of T9002 in M9/Yeast in three tubes of 11x, 22x and 44x.
