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EPF-Lausanne/14 August 2008
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All mini-preps of F1610 have a fragment of 300 bp. So the biobrick from IGEM doesn't work. | All mini-preps of F1610 have a fragment of 300 bp. So the biobrick from IGEM doesn't work. | ||
All ligated fragments E1010/F1610 aren't ligate. | All ligated fragments E1010/F1610 aren't ligate. | ||
| - | (see | + | |
| + | (see Gel picture on page 75 in notebook of lab) | ||
==Test of specific primer of BBs== | ==Test of specific primer of BBs== | ||
We do a PCR to see if specific primers that Alexandre commanded work with biobrick. | We do a PCR to see if specific primers that Alexandre commanded work with biobrick. | ||
We do gel tomorrow. | We do gel tomorrow. | ||
Revision as of 16:07, 14 August 2008
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Molecular biology
We do mini-prep of E1010, "F1610" and P0140
E1010/F1610
Yesterday we find that F1610 make 300bp. So two hypothesis: 1) The mini-prep is bad or mix 2) the biobrick from IGEM is bad.
So we do another digestion serie with EcoRI and SpeI. We do with different mini-prep of F1610 and the last mini-prep of E1010 that work(for positiv control).
Some mini-prep from F1610 are too small, there isn't enough liquid so we do PCR with these (we use E1010 from last mini-prep as a positiv control).
We do in same time PCR of all fragments that ligated E1010/F1610 to see if there is a fragment of 1500 bp.
Resultat of Gel
All mini-preps of F1610 have a fragment of 300 bp. So the biobrick from IGEM doesn't work. All ligated fragments E1010/F1610 aren't ligate.
(see Gel picture on page 75 in notebook of lab)
Test of specific primer of BBs
We do a PCR to see if specific primers that Alexandre commanded work with biobrick. We do gel tomorrow.
