User:University of Washington/13 August 2008

From 2008.igem.org

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(LuxR from AraC and TetR(Faifan))
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<table>
<table>
<tr><th></th><th>dH2O(ul)</th><th>Buffer(ul)</th><th>BSA(ul)</th><th>DNA(ul)</th><th>E(ul)</th><th>X(ul)</th><th>X(ul)</th><th>S(ul)</th><th>P(ul)</th></tr>
<tr><th></th><th>dH2O(ul)</th><th>Buffer(ul)</th><th>BSA(ul)</th><th>DNA(ul)</th><th>E(ul)</th><th>X(ul)</th><th>X(ul)</th><th>S(ul)</th><th>P(ul)</th></tr>
-
<tr><th>BBa_Elowitz</th><th>32.5</th><th>EcoRI, 5</th><th>0.5</th><th>10</th><th>1</th><th>-</th><th>1</th><th>-</th></tr>
+
<tr><td>BBa_Elowitz</td><td>32.5</td><td>EcoRI, 5</td><td>0.5</td><td>10</td><td>1</td><td>-</td><td>1</td><td>-</td></tr>
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<tr><th>LuxR</th><th>32.5</th><th>NEB3, 5</th><th>0.5</th><th>10</th><th>-</th><th>1</th><th>-</th><th>1</th></tr>
+
<tr><td>LuxR</td><td>32.5</td><td>NEB3, 5</td><td>0.5</td><td>10</td><td>-</td><td>1</td><td>-</td><td>1</td></tr>
-
<tr><th>GFP</th><th>32.5</th><th>NEB3, 5</th><th>0.5</th><th>10</th><th>-</th><th>1</th><th>-</th><th>1</th></tr>
+
<tr><td>GFP</td><td>32.5</td><td>NEB3, 5</td><td>0.5</td><td>10</td><td>-</td><td>1</td><td>-</td><td>1</td></tr>
-
<tr><th>P1010</th><th>32.5</th><th>EcoRI, 5</th><th>0.5</th><th>10</th><th>1</th><th>-</th><th>-</th><th>1</th></tr>
+
<tr><td>P1010</td><td>32.5</td><td>EcoRI, 5</td><td>0.5</td><td>10</td><td>1</td><td>-</td><td>-</td><td>1</td></tr>
</table>
</table>

Revision as of 22:02, 14 August 2008

Contents

Conjugation (Scott)

experiment from Aug. 7 results

experiment #BacteriaYeastYeast counttransconjugation efficiency
1250uL250uL662.64E-5
2200uL300uL642.13E-5
3100uL400uL328.00e-6
450uL450uL184.00e-6

LuxR from AraC and TetR(Faifan)

QuikChange Approach

- Overnight QuikChange#4 culture from Amp plate (reaction1 x 4, reaction2 x 2) in Tsy+Amp

- Sequence of DNA from QuikChange#3 came back ==> No mutation.

Elowitz's

-Restriction Digest of BBa_Elowitz, BBa_E0240(GFP), BBa_I0462(LuxR), BBa_P1010(on pSB3K3)

dH2O(ul)Buffer(ul)BSA(ul)DNA(ul)E(ul)X(ul)X(ul)S(ul)P(ul)
BBa_Elowitz32.5EcoRI, 50.5101-1-
LuxR32.5NEB3, 50.510-1-1
GFP32.5NEB3, 50.510-1-1
P101032.5EcoRI, 50.5101--1




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