Team:Paris/August 15

From 2008.igem.org

(Difference between revisions)
(→Starting the construction of the Promoter characterization plasmid)
(→Starting the construction of the Promoter characterization plasmid)
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=Starting the construction of the Promoter characterization plasmid=
=Starting the construction of the Promoter characterization plasmid=
-
[[Team:Paris/Notebook/Protocols#Migration after digestion|standard protocol]]
 
-
===Digestion===
+
==Digestion==
-
==Measurement of concentration of minipreps==
+
=Measurement of concentration of minipreps=
[[Team:Paris/Notebook/Protocols#Concentration of the Miniprep|standard protocol]]
[[Team:Paris/Notebook/Protocols#Concentration of the Miniprep|standard protocol]]
{| border="1" style="text-align: center"
{| border="1" style="text-align: center"
-
|Digestion
+
|Plasmid
-
|Miniprep used
+
|Miniprep
|Concentration (µg/mL)
|Concentration (µg/mL)
|ratio 260/280
|ratio 260/280
|-
|-
-
|D146
+
|MP3
-
|MP148.2
+
|3
-
|123
+
|152
-
|1.58
+
|1.43
|-
|-
-
|D147
+
|MP3
-
|MP153.3
+
|4
-
|113
+
|775
-
|1.68
+
|1.21
 +
|-
 +
|MP101
 +
|1
 +
|317
 +
|1.66
 +
|-
 +
|MP101
 +
|2
 +
|389
 +
|1.36
 +
|-
 +
|MP101
 +
|4
 +
|209
 +
|1.76
 +
|-
 +
|MP104
 +
|1
 +
|173
 +
|1.32
 +
|-
 +
|MP104
 +
|3
 +
|43
 +
|1.85
 +
|-
 +
|MP104
 +
|4
 +
|52
 +
|1.66
 +
|-
 +
|MP114
 +
|1
 +
|173
 +
|1.75
 +
|-
 +
|MP114
 +
|2
 +
|263
 +
|1.43
 +
|-
 +
|MP143
 +
|1
 +
|133
 +
|1.55
 +
|-
 +
|MP143
 +
|2
 +
|132
 +
|1.70
|}
|}
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{| border="1" style="text-align: center"
{| border="1" style="text-align: center"
-
|Digestion
+
|Plasmid
 +
|Description
|Miniprep used
|Miniprep used
-
|Concentration (µg/mL)
+
|Enzymes
-
|ratio 260/280
+
|-
|-
-
|D146
+
|MP3
-
|MP148.2
+
|
|123
|123
|1.58
|1.58

Revision as of 16:05, 15 August 2008

← Yesterday

↓ Calendar ↑

Tomorrow →

Contents

Transformation of the ligations we did yesterday

We transformed L 143, L 144 and the negative controls T1 and T2, using Invitrogen's TOP10 chemically competent cells
standard protocol.


Digestion

Measurement of concentration of minipreps

standard protocol

Digestion Miniprep used Concentration (µg/mL) ratio 260/280
D146 MP148.2 123 1.58
D147 MP153.3 113 1.68


Digestion

Protocol Digestion


Starting the construction of the Promoter characterization plasmid

Digestion

Measurement of concentration of minipreps

standard protocol

Plasmid Miniprep Concentration (µg/mL) ratio 260/280
MP3 3 152 1.43
MP3 4 775 1.21
MP101 1 317 1.66
MP101 2 389 1.36
MP101 4 209 1.76
MP104 1 173 1.32
MP104 3 43 1.85
MP104 4 52 1.66
MP114 1 173 1.75
MP114 2 263 1.43
MP143 1 133 1.55
MP143 2 132 1.70

Digestion

Protocol Digestion

Plasmid Description Miniprep used Enzymes
MP3 123 1.58
D147 MP153.3 113 1.68

Analysis of yesterday PCR screening

KR000163.jpg KR000162.jpg KR000164.jpg

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