Template:Team:UC Berkeley/Notebook/SC Asequencing
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+ | <span style='font-family:"Century Gothic";color:maroon;font-size:10.0pt' align="center"> | ||
+ | <div style="text-align: right;"> [[Team:UC_Berkeley/Notebook/Sherine_Cheung|Back to Sherine's Notebook]] </div> | ||
+ | <div style="text-align: right;"> [[Team:UC_Berkeley/Notebook| To All Notebooks]] </div> | ||
+ | |||
{| cellpadding="3" cellspacing="5" border="1" | {| cellpadding="3" cellspacing="5" border="1" | ||
- | | '''''Read''''' || '''''Date''''' || ''''' | + | | '''''Read''''' || '''''Date''''' || '''''sca #''''' || '''''Clone #''''' || '''''Oligo'''''|| '''''Result''''' || '''''File''''' |
|- | |- | ||
- | | CC09 | + | | CC09 || 7/18/2008 || SCA15(trial 1)|| Clone #1 || ca998/g00101 || Bad read || [[SCA15]] |
|- | |- | ||
| CC10 || 7/18/2008 || SCA17(trial 1)|| Clone #2 || ca998/g00101 || Bad read || [[SCA17]] | | CC10 || 7/18/2008 || SCA17(trial 1)|| Clone #2 || ca998/g00101 || Bad read || [[SCA17]] | ||
Line 10: | Line 14: | ||
| CC12 || 7/22/2008 || SCA19(trial 1) || Clone #1 || ca998 || Low signal || [[SCA19]] | | CC12 || 7/22/2008 || SCA19(trial 1) || Clone #1 || ca998 || Low signal || [[SCA19]] | ||
|- | |- | ||
- | | CC13 || 7/22/2008 || SCA18(trial 1) || Clone #4 || ca998 || Low signal || | + | | CC13 || 7/22/2008 || SCA18(trial 1) || Clone #4 || ca998 || Low signal || [[SCA18-2]] |
|- | |- | ||
- | | CC14 || 7/22/2008 || SCA19(trial 1) || Clone #1 || ca998 || Low signal || | + | | CC14 || 7/22/2008 || SCA19(trial 1) || Clone #1 || ca998 || Low signal || [[SCA19-2]] |
|- | |- | ||
| SC16 || 7/22/2008 || SCA16 || Clone #3 || ca998 || Bad--mixed inserts || [[SCA16]] | | SC16 || 7/22/2008 || SCA16 || Clone #3 || ca998 || Bad--mixed inserts || [[SCA16]] | ||
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| SC21 || 7/22/2008 || SCA25-1 || Clone #1 || ca998 || Perfect || [[SCA25]] | | SC21 || 7/22/2008 || SCA25-1 || Clone #1 || ca998 || Perfect || [[SCA25]] | ||
|- | |- | ||
- | | SC22 || 7/23/2008 || sca3-2 (fr. SP) || Clone #2 || ca998 || Did not work || | + | | SC22 || 7/23/2008 || sca3-2 (fr. SP) || Clone #2 || ca998 || Did not work || [[SCA3-SP2]] |
|- | |- | ||
- | | SC23 || 7/24/2008 || sca3-PR2 || Clone #2 || ca998 || mixed inserts || | + | | SC23 || 7/24/2008 || sca3-PR2 || Clone #2 || ca998 || mixed inserts || [[SCA3-PR2]] |
|- | |- | ||
- | | SC24 || 7/24/2008 || | + | | SC24 || 7/24/2008 || sca15-1 (trial 2) || Clone #1 || ca998/g00101 ||ca998 weird;g00101 good || [[SCA15-1]] |
|- | |- | ||
- | | SC25 || 7/ | + | | SC25 || 7/30/2008 || sca1 T || Clone # || ca998/g00101 || 2 pt mutations || [[SC25]] |
|- | |- | ||
- | | SC26 || 7/ | + | | SC26 || 7/30/2008 || sca2 T || Clone # || ca998 || Bad Read || [[SC26]] |
|- | |- | ||
- | | SC27 || 7/ | + | | SC27 || 7/30/2008 || sca3 T || Clone #1 || ca998 || Bad Read || [[SC27]] |
|- | |- | ||
- | | SC28 || 7/ | + | | SC28 || 7/30/2008 || sca4 T || Clone # || ca998 || Perfect(missing EcoRI) || [[SC28]] |
|- | |- | ||
- | | SC29 || 7/ | + | | SC29 || 7/30/2008 || sca6 T || Clone # || ca998 || Perfect || [[SC29]] |
|- | |- | ||
- | | SC30 || 7/ | + | | SC30 || 7/30/2008 || sca7 T || Clone # || ca998 || Perfect || [[SC30]] |
|- | |- | ||
- | | SC31 || 7/ | + | | SC31 || 7/30/2008 || sca8 T || Clone # || ca998/g00101 || Perfect || [[SC31]] |
|- | |- | ||
- | | SC32 || 7/ | + | | SC32 || 7/30/2008 || sca9 T || Clone # || ca998/g00101 || random sequence || [[SC32]] |
|- | |- | ||
- | | SC33 || 7/ | + | | SC33 || 7/30/2008 || sca14 T || Clone # || ca998 || Perfect || [[SC33]] |
+ | |- | ||
+ | | CC15 || 7/29/2008 || sca5 (pBca1256) || Clone #1 || ca998 || Perfect || [[SCA5-1]] | ||
+ | |- | ||
+ | | CC16 || 7/29/2008 || sca5 (pBca1256) || Clone #2 || ca998 || Perfect || [[SCA5-2]] | ||
+ | |- | ||
+ | | CC17 || 7/29/2008 || sca11 (pBca1256) || Clone #1 || ca998 || Perfect || [[SCA11-1]] | ||
+ | |- | ||
+ | | CC18 || 7/29/2008 || sca11 (pBca1256)|| Clone #2 || ca998 || Perfect || [[SCA11-2]] | ||
+ | |- | ||
+ | | CC19 || 8/1/2008 || sca5 T || Clone #2 || ca998 || Perfect. no Bam site || [[SCA5 T2]] | ||
+ | |- | ||
+ | | CC20 || 8/1/2008 || sca11 T || Clone #1 || ca998 || Perfect || [[SCA11 T1]] | ||
+ | |- | ||
+ | | CC21 (CC11)|| 8/3/2008 || sca5 T {<HA!} || Clone #2 || G00101 || Perfect || [[sca5 T]] | ||
+ | |- | ||
+ | | CC22 (CC12)|| 8/3/2008 || sca11 T {<HA>}|| Clone #1 || G00101 || Perfect || [[sca11 T]] | ||
+ | |- | ||
+ | | SC42 || 8/4/2008 || sca2-1 || Clone #1 || g00101(try) || no part...wrong || [[sca2-1]] | ||
+ | |- | ||
+ | | SC43 || Date || sca3-9 || Clone #9 || ca998(&RS) || Bad Read || file | ||
+ | |- | ||
+ | | SC44 || 8/5/2008 || sca23-4 || Clone #4 || ca998 || Perfect || [[sca23-4]] | ||
+ | |- | ||
+ | | SC45 || 8//8/2008 ||Stock amplif. fimH || 1 sample || ca998 || mixed inserts || [[Amplif. fimH]] | ||
+ | |- | ||
+ | | <s>SC46</s> || Date || fimH || Clone # || oligo || result || file | ||
+ | |- | ||
+ | | SC47 || 8/13/2008 || sca40-1 || Clone #1 || g00101 || Perfect || [[sca40-1]] | ||
+ | |- | ||
+ | | SC48 || 8/13/2008 || sca40-4 || Clone #4 || g00101 || mixed inserts || [[sca40-4]] | ||
+ | |- | ||
+ | | SC49 || 8/13/2008 || sca41-4 || Clone #4 || g00101 || Perfect || [[sca41-4]] | ||
+ | |- | ||
+ | | SC50 || 8/13/2008 || sca42-2 || Clone #2 || g00101 || short - partial perfect || [[sca42-2]] | ||
+ | |- | ||
+ | | SC51 || 8/13/2008|| sca42-5 (maybe sca43-2)? || Clone #5 || g00101 || Perfect(but contains <FLAG!) || [[sca42-5]] | ||
+ | |- | ||
+ | | SC52 || 8/13/2008 || sca43-4 || Clone #4 || g00101 || short - only b1006 present || [[sca43-4]] | ||
+ | |- | ||
+ | | SC53 || 8/14/2008 || sca42-5 || Clone #5 || g00101 || mixed inserts || [[sca42-5 2nd try]] | ||
+ | |- | ||
+ | | SC54 || 8/14/2008 || sca43-2 || Clone #2 || g00101 || perfect || [[sca43-2]] | ||
+ | |- | ||
+ | | SC55 || 8/14/2008 || sca44 || Clone #1 || ca998 || perfect || [[sca44-1]] | ||
+ | |- | ||
+ | | SC56 || 8/14/2008 || sca45 || Clone #1 || ca998 || perfect || [[sca45-1]] | ||
+ | |- | ||
+ | | SC57 || 8/14/2008 || sca46 || Clone #1 || ca998 || perfect || [[sca46-1]] | ||
+ | |- | ||
+ | | SC58 || 8/14/2008 || sca47 || Clone #1 || ca998 || perfect (2nd try) || [[sca47-1]] | ||
+ | |- | ||
+ | | SC59 || 8/15/2008 || sca42 || Clone #7 || g00101 || short, missing phoA || [[sca42-7]] | ||
+ | |- | ||
+ | | SC60 || 8/15/2008 || sca42 || Clone #8 || g00101 || short, perfect || [[sca42-8]] | ||
+ | |- | ||
+ | | SC61 || 8/15/2008 || sca42 || Clone #12 || g00101 || short, perfect || [[sca42-12]] | ||
+ | |- | ||
+ | | Read || Date || Description || Clone # || oligo || result || file | ||
+ | |- | ||
+ | | Read || Date || Description || Clone # || oligo || result || file | ||
+ | |- | ||
+ | | Read || Date || Description || Clone # || oligo || result || file | ||
+ | |- | ||
+ | | Read || Date || Description || Clone # || oligo || result || file | ||
+ | |- | ||
+ | | Read || Date || Description || Clone # || oligo || result || file | ||
+ | |- | ||
+ | | Read || Date || Description || Clone # || oligo || result || file | ||
|- | |- | ||
|} | |} |
Latest revision as of 20:06, 16 August 2008
Read | Date | sca # | Clone # | Oligo | Result | File |
CC09 | 7/18/2008 | SCA15(trial 1) | Clone #1 | ca998/g00101 | Bad read | SCA15 |
CC10 | 7/18/2008 | SCA17(trial 1) | Clone #2 | ca998/g00101 | Bad read | SCA17 |
CC11 | 7/22/2008 | SCA18(trial 1) | Clone #4 | ca998 | Low signal | SCA18 |
CC12 | 7/22/2008 | SCA19(trial 1) | Clone #1 | ca998 | Low signal | SCA19 |
CC13 | 7/22/2008 | SCA18(trial 1) | Clone #4 | ca998 | Low signal | SCA18-2 |
CC14 | 7/22/2008 | SCA19(trial 1) | Clone #1 | ca998 | Low signal | SCA19-2 |
SC16 | 7/22/2008 | SCA16 | Clone #3 | ca998 | Bad--mixed inserts | SCA16 |
SC17 | 7/22/2008 | SCA20 | Clone #5 | ca998/G00101 | Bad--mixed inserts | SCA20 |
SC18 | 7/22/2008 | SCA22-1 | Clone #1 | ca998 | Perfect | SCA22 |
SC19 | 7/22/2008 | SCA23-3 | Clone #3 | ca998 | Perfect | SCA23 |
SC20 | 7/22/2008 | SCA24-1 | Clone #1 | ca998 | Perfect | SCA24 |
SC21 | 7/22/2008 | SCA25-1 | Clone #1 | ca998 | Perfect | SCA25 |
SC22 | 7/23/2008 | sca3-2 (fr. SP) | Clone #2 | ca998 | Did not work | SCA3-SP2 |
SC23 | 7/24/2008 | sca3-PR2 | Clone #2 | ca998 | mixed inserts | SCA3-PR2 |
SC24 | 7/24/2008 | sca15-1 (trial 2) | Clone #1 | ca998/g00101 | ca998 weird;g00101 good | SCA15-1 |
SC25 | 7/30/2008 | sca1 T | Clone # | ca998/g00101 | 2 pt mutations | SC25 |
SC26 | 7/30/2008 | sca2 T | Clone # | ca998 | Bad Read | SC26 |
SC27 | 7/30/2008 | sca3 T | Clone #1 | ca998 | Bad Read | SC27 |
SC28 | 7/30/2008 | sca4 T | Clone # | ca998 | Perfect(missing EcoRI) | SC28 |
SC29 | 7/30/2008 | sca6 T | Clone # | ca998 | Perfect | SC29 |
SC30 | 7/30/2008 | sca7 T | Clone # | ca998 | Perfect | SC30 |
SC31 | 7/30/2008 | sca8 T | Clone # | ca998/g00101 | Perfect | SC31 |
SC32 | 7/30/2008 | sca9 T | Clone # | ca998/g00101 | random sequence | SC32 |
SC33 | 7/30/2008 | sca14 T | Clone # | ca998 | Perfect | SC33 |
CC15 | 7/29/2008 | sca5 (pBca1256) | Clone #1 | ca998 | Perfect | SCA5-1 |
CC16 | 7/29/2008 | sca5 (pBca1256) | Clone #2 | ca998 | Perfect | SCA5-2 |
CC17 | 7/29/2008 | sca11 (pBca1256) | Clone #1 | ca998 | Perfect | SCA11-1 |
CC18 | 7/29/2008 | sca11 (pBca1256) | Clone #2 | ca998 | Perfect | SCA11-2 |
CC19 | 8/1/2008 | sca5 T | Clone #2 | ca998 | Perfect. no Bam site | SCA5 T2 |
CC20 | 8/1/2008 | sca11 T | Clone #1 | ca998 | Perfect | SCA11 T1 |
CC21 (CC11) | 8/3/2008 | sca5 T {<HA!} | Clone #2 | G00101 | Perfect | sca5 T |
CC22 (CC12) | 8/3/2008 | sca11 T {<HA>} | Clone #1 | G00101 | Perfect | sca11 T |
SC42 | 8/4/2008 | sca2-1 | Clone #1 | g00101(try) | no part...wrong | sca2-1 |
SC43 | Date | sca3-9 | Clone #9 | ca998(&RS) | Bad Read | file |
SC44 | 8/5/2008 | sca23-4 | Clone #4 | ca998 | Perfect | sca23-4 |
SC45 | 8//8/2008 | Stock amplif. fimH | 1 sample | ca998 | mixed inserts | Amplif. fimH |
| Date | fimH | Clone # | oligo | result | file |
SC47 | 8/13/2008 | sca40-1 | Clone #1 | g00101 | Perfect | sca40-1 |
SC48 | 8/13/2008 | sca40-4 | Clone #4 | g00101 | mixed inserts | sca40-4 |
SC49 | 8/13/2008 | sca41-4 | Clone #4 | g00101 | Perfect | sca41-4 |
SC50 | 8/13/2008 | sca42-2 | Clone #2 | g00101 | short - partial perfect | sca42-2 |
SC51 | 8/13/2008 | sca42-5 (maybe sca43-2)? | Clone #5 | g00101 | Perfect(but contains <FLAG!) | sca42-5 |
SC52 | 8/13/2008 | sca43-4 | Clone #4 | g00101 | short - only b1006 present | sca43-4 |
SC53 | 8/14/2008 | sca42-5 | Clone #5 | g00101 | mixed inserts | sca42-5 2nd try |
SC54 | 8/14/2008 | sca43-2 | Clone #2 | g00101 | perfect | sca43-2 |
SC55 | 8/14/2008 | sca44 | Clone #1 | ca998 | perfect | sca44-1 |
SC56 | 8/14/2008 | sca45 | Clone #1 | ca998 | perfect | sca45-1 |
SC57 | 8/14/2008 | sca46 | Clone #1 | ca998 | perfect | sca46-1 |
SC58 | 8/14/2008 | sca47 | Clone #1 | ca998 | perfect (2nd try) | sca47-1 |
SC59 | 8/15/2008 | sca42 | Clone #7 | g00101 | short, missing phoA | sca42-7 |
SC60 | 8/15/2008 | sca42 | Clone #8 | g00101 | short, perfect | sca42-8 |
SC61 | 8/15/2008 | sca42 | Clone #12 | g00101 | short, perfect | sca42-12 |
Read | Date | Description | Clone # | oligo | result | file |
Read | Date | Description | Clone # | oligo | result | file |
Read | Date | Description | Clone # | oligo | result | file |
Read | Date | Description | Clone # | oligo | result | file |
Read | Date | Description | Clone # | oligo | result | file |
Read | Date | Description | Clone # | oligo | result | file |