PCR products

From 2008.igem.org

(Difference between revisions)
(New page: <html> <head> <title>Edinburgh iGem 2008</title> <script type="text/javascript"> //Drop Down Tabs Menu- Author: Dynamic Drive (http://www.dynamicdrive.com) //Created: May 16th, 07' var ta...)
(Removing all content from page)
 
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<html>
 
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<head>
 
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<title>Edinburgh iGem 2008</title>
 
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<script type="text/javascript">
 
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//Drop Down Tabs Menu- Author: Dynamic Drive (http://www.dynamicdrive.com)
 
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//Created: May 16th, 07'
 
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var tabdropdown={
 
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* disappeardelay: 200, //set delay in miliseconds before menu disappears onmouseout
 
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disablemenuclick: false, //when user clicks on a menu item with a drop down menu, disable menu item's link?
 
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enableiframeshim: 1, //1 or 0, for true or false
 
-
 
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//No need to edit beyond here////////////////////////
 
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dropmenuobj: null, ie: document.all, firefox: document.getElementById&&!document.all, previousmenuitem:null,
 
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currentpageurl: window.location.href.replace("http://"+window.location.hostname, "").replace(/^\//, ""), //get current page url (minus hostname, ie: http://www.dynamicdrive.com/)
 
-
 
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getposOffset:function(what, offsettype){
 
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var totaloffset=(offsettype=="left")? what.offsetLeft : what.offsetTop;
 
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var parentEl=what.offsetParent;
 
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while (parentEl!=null){
 
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totaloffset=(offsettype=="left")? totaloffset+parentEl.offsetLeft : totaloffset+parentEl.offsetTop;
 
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parentEl=parentEl.offsetParent;
 
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}
 
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return totaloffset;
 
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},
 
-
 
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showhide:function(obj, e, obj2){ //obj refers to drop down menu, obj2 refers to tab menu item mouse is currently over
 
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if (this.ie || this.firefox)
 
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this.dropmenuobj.style.left=this.dropmenuobj.style.top="-500px"
 
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if (e.type=="click" && obj.visibility==hidden || e.type=="mouseover"){
 
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if (obj2.parentNode.className.indexOf("default")==-1) //if tab isn't a default selected one
 
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obj2.parentNode.className="selected"
 
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obj.visibility="visible"
 
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}
 
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else if (e.type=="click")
 
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obj.visibility="hidden"
 
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},
 
-
 
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iecompattest:function(){
 
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return (document.compatMode && document.compatMode!="BackCompat")? document.documentElement : document.body
 
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},
 
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clearbrowseredge:function(obj, whichedge){
 
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var edgeoffset=0
 
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if (whichedge=="rightedge"){
 
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var windowedge=this.ie && !window.opera? this.standardbody.scrollLeft+this.standardbody.clientWidth-15 : window.pageXOffset+window.innerWidth-15
 
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this.dropmenuobj.contentmeasure=this.dropmenuobj.offsetWidth
 
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if (windowedge-this.dropmenuobj.x < this.dropmenuobj.contentmeasure)  //move menu to the left?
 
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edgeoffset=this.dropmenuobj.contentmeasure-obj.offsetWidth
 
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}
 
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else{
 
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var topedge=this.ie && !window.opera? this.standardbody.scrollTop : window.pageYOffset
 
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var windowedge=this.ie && !window.opera? this.standardbody.scrollTop+this.standardbody.clientHeight-15 : window.pageYOffset+window.innerHeight-18
 
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this.dropmenuobj.contentmeasure=this.dropmenuobj.offsetHeight
 
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if (windowedge-this.dropmenuobj.y < this.dropmenuobj.contentmeasure){ //move up?
 
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edgeoffset=this.dropmenuobj.contentmeasure+obj.offsetHeight
 
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if ((this.dropmenuobj.y-topedge)<this.dropmenuobj.contentmeasure) //up no good either?
 
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edgeoffset=this.dropmenuobj.y+obj.offsetHeight-topedge
 
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}
 
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this.dropmenuobj.firstlink.style.borderTopWidth=(edgeoffset==0)? 0 : "1px" //Add 1px top border to menu if dropping up
 
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}
 
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return edgeoffset
 
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},
 
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dropit:function(obj, e, dropmenuID){
 
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if (this.dropmenuobj!=null){ //hide previous menu
 
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this.dropmenuobj.style.visibility="hidden" //hide menu
 
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if (this.previousmenuitem!=null && this.previousmenuitem!=obj){
 
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if (this.previousmenuitem.parentNode.className.indexOf("default")==-1) //If the tab isn't a default selected one
 
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this.previousmenuitem.parentNode.className=""
 
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}
 
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}
 
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this.clearhidemenu()
 
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if (this.ie||this.firefox){
 
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obj.onmouseout=function(){tabdropdown.delayhidemenu(obj)}
 
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obj.onclick=function(){return !tabdropdown.disablemenuclick} //disable main menu item link onclick?
 
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this.dropmenuobj=document.getElementById(dropmenuID)
 
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this.dropmenuobj.onmouseover=function(){tabdropdown.clearhidemenu()}
 
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this.dropmenuobj.onmouseout=function(e){tabdropdown.dynamichide(e, obj)}
 
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this.dropmenuobj.onclick=function(){tabdropdown.delayhidemenu(obj)}
 
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this.showhide(this.dropmenuobj.style, e, obj)
 
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this.dropmenuobj.x=this.getposOffset(obj, "left")
 
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this.dropmenuobj.y=this.getposOffset(obj, "top")
 
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this.dropmenuobj.style.left=this.dropmenuobj.x-this.clearbrowseredge(obj, "rightedge")+"px"
 
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this.dropmenuobj.style.top=this.dropmenuobj.y-this.clearbrowseredge(obj, "bottomedge")+obj.offsetHeight+1+"px"
 
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this.previousmenuitem=obj //remember main menu item mouse moved out from (and into current menu item)
 
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this.positionshim() //call iframe shim function
 
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}
 
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},
 
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contains_firefox:function(a, b) {
 
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while (b.parentNode)
 
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if ((b = b.parentNode) == a)
 
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return true;
 
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return false;
 
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},
 
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dynamichide:function(e, obj2){ //obj2 refers to tab menu item mouse is currently over
 
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var evtobj=window.event? window.event : e
 
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if (this.ie&&!this.dropmenuobj.contains(evtobj.toElement))
 
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this.delayhidemenu(obj2)
 
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else if (this.firefox&&e.currentTarget!= evtobj.relatedTarget&& !this.contains_firefox(evtobj.currentTarget, evtobj.relatedTarget))
 
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this.delayhidemenu(obj2)
 
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},
 
-
 
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delayhidemenu:function(obj2){
 
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this.delayhide=setTimeout(function(){tabdropdown.dropmenuobj.style.visibility='hidden'; if (obj2.parentNode.className.indexOf('default')==-1) obj2.parentNode.className=''},this.disappeardelay) //hide menu
 
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},
 
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clearhidemenu:function(){
 
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if (this.delayhide!="undefined")
 
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clearTimeout(this.delayhide)
 
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},
 
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positionshim:function(){ //display iframe shim function
 
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if (this.enableiframeshim && typeof this.shimobject!="undefined"){
 
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if (this.dropmenuobj.style.visibility=="visible"){
 
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this.shimobject.style.width=this.dropmenuobj.offsetWidth+"px"
 
-
this.shimobject.style.height=this.dropmenuobj.offsetHeight+"px"
 
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this.shimobject.style.left=this.dropmenuobj.style.left
 
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this.shimobject.style.top=this.dropmenuobj.style.top
 
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}
 
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this.shimobject.style.display=(this.dropmenuobj.style.visibility=="visible")? "block" : "none"
 
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}
 
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},
 
-
 
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hideshim:function(){
 
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if (this.enableiframeshim && typeof this.shimobject!="undefined")
 
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this.shimobject.style.display='none'
 
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},
 
-
 
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isSelected:function(menuurl){
 
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var menuurl=menuurl.replace("http://"+menuurl.hostname, "").replace(/^\//, "")
 
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return (tabdropdown.currentpageurl==menuurl)
 
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},
 
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init:function(menuid, dselected){
 
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this.standardbody=(document.compatMode=="CSS1Compat")? document.documentElement : document.body //create reference to common "body" across doctypes
 
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var menuitems=document.getElementById(menuid).getElementsByTagName("a")
 
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for (var i=0; i<menuitems.length; i++){
 
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if (menuitems[i].getAttribute("rel")){
 
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var relvalue=menuitems[i].getAttribute("rel")
 
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document.getElementById(relvalue).firstlink=document.getElementById(relvalue).getElementsByTagName("a")[0]
 
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menuitems[i].onmouseover=function(e){
 
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var event=typeof e!="undefined"? e : window.event
 
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tabdropdown.dropit(this, event, this.getAttribute("rel"))
 
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}
 
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}
 
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if (dselected=="auto" && typeof setalready=="undefined" && this.isSelected(menuitems[i].href)){
 
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menuitems[i].parentNode.className+=" selected default"
 
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var setalready=true
 
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}
 
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else if (parseInt(dselected)==i)
 
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menuitems[i].parentNode.className+=" selected default"
 
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}
 
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}
 
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}
 
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</script>
 
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<style>
 
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.ddcolortabs{
 
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padding: 0;
 
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width: 100%;
 
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background: transparent;
 
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voice-family: "\"}\"";
 
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voice-family: inherit;
 
-
}
 
-
 
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.ddcolortabs ul{
 
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font: normal 11px Arial, Verdana, sans-serif;
 
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margin:0;
 
-
padding:0;
 
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list-style:none;
 
-
}
 
-
 
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.ddcolortabs li{
 
-
display:inline;
 
-
margin:0 2px 0 0;
 
-
padding:0;
 
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text-transform:uppercase;
 
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}
 
-
 
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.ddcolortabs a{
 
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float:left;
 
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color: white;
 
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background: black url(media/color_tabs_left.gif) no-repeat left top;
 
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margin:0 2px 0 0;
 
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padding:0 0 1px 3px;
 
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text-decoration:none;
 
-
letter-spacing: 1px;
 
-
}
 
-
 
-
.ddcolortabs a span{
 
-
float:left;
 
-
display:block;
 
-
background: transparent url(media/color_tabs_right.gif) no-repeat right top;
 
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padding: 4px 8px 2px 7px;
 
-
}
 
-
 
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.ddcolortabs a span{
 
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float:none;
 
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}
 
-
 
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.ddcolortabs a:hover{
 
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background-color: #591f20;
 
-
}
 
-
 
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.ddcolortabs a:hover span{
 
-
background-color: #591f20;
 
-
}
 
-
 
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.ddcolortabs .selected a, #ddcolortabs .selected a span{ /*currently selected tab*/
 
-
background-color: #591f20;
 
-
}
 
-
 
-
.ddcolortabsline{
 
-
clear: both;
 
-
padding: 0;
 
-
width: 100%;
 
-
height: 8px;
 
-
line-height: 8px;
 
-
background: black;
 
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border-top: 1px solid #fff; /*Remove this to remove border between bar and tabs*/
 
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}
 
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/* ######### Style for Drop Down Menu ######### */
 
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.dropmenudiv_a{
 
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position:absolute;
 
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top: 0;
 
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border: 1px solid black; /*THEME CHANGE HERE*/
 
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border-top-width: 8px; /*Top border width. Should match height of .ddcolortabsline above*/
 
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border-bottom-width: 0;
 
-
font:normal 12px Arial;
 
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line-height:18px;
 
-
z-index:100;
 
-
background-color: white;
 
-
width: 200px;
 
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visibility: hidden;
 
-
}
 
-
 
-
 
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.dropmenudiv_a a{
 
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width: auto;
 
-
display: block;
 
-
text-indent: 5px;
 
-
border-top: 0 solid #678b3f;
 
-
border-bottom: 1px solid #678b3f; /*THEME CHANGE HERE*/
 
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padding: 2px 0;
 
-
text-decoration: none;
 
-
color: black;
 
-
}
 
-
 
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* html .dropmenudiv_a a{ /*IE only hack*/
 
-
width: 100%;
 
-
}
 
-
 
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.dropmenudiv_a a:hover{ /*THEME CHANGE HERE*/
 
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background-color: #8a3c3d;
 
-
color: white;
 
-
}
 
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</style>
 
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</head>
 
-
 
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<!-- CSS for Drop Down Tabs Menu #1 -->
 
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<link rel="stylesheet" type="text/css" href="ddcolortabs.css" />
 
-
 
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<div id="colortab" class="ddcolortabs">
 
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<ul>
 
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<li><a href="https://2008.igem.org/Team:Edinburgh" title="Home"><span>Home</span></a></li>
 
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<li><a href="https://2008.igem.org/Team:Edinburgh/Project" title="Project" rel="dropmenu1_a"><span>The Project</span></a></li>
 
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<li><a href="https://2008.igem.org/Team:Edinburgh/Team" title="Team" ><span>The Team</span></a></li>
 
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<li><a href="http://partsregistry.org/cgi/partsdb/pgroup.cgi?pgroup=iGEM2008&group=Edinburgh" title="Project" rel="dropmenu1_a"><span>BioBrick Parts</span></a></li>
 
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<li><a href="https://2008.igem.org/Team:Edinburgh/Modeling" title="Modelling" rel="dropmenu2_a"><span>Modelling</span></a></li>
 
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<li><a href="https://2008.igem.org/Team:Edinburgh/Notebook" title="Notebook"><span>Notebook</span></a></li>
 
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</ul>
 
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</div>
 
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<div class="ddcolortabsline">&nbsp;</div>
 
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<div id="dropmenu1_a" class="dropmenudiv_a">
 
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<a href="https://2008.igem.org/Team:Edinburgh/Team">Overview</a>
 
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<a href="https://2008.igem.org/Team:Edinburgh/Team">Step1</a>
 
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<a href="https://2008.igem.org/Team:Edinburgh/Team">Step2</a>
 
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</div>
 
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<script type="text/javascript">
 
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//SYNTAX: tabdropdown.init("menu_id", [integer OR "auto"])
 
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tabdropdown.init("colortab", 3)
 
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</script>
 
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</html>
 
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'''P1:''' ''dxs'' from ''Eschaerichia coli'' JM109 (or K12?)<br />
 
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'''P2:''' ''appY'' from ''E. coli'' JM109 (or K12?)<br />
 
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'''P3:''' ''glgC'' from ''E. coli'' JM109 (or K12?)<br />
 
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'''P4:''' fusion PCR for BABEL1+''glgC''<br />
 
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'''P5:''' fusion PCR for BABEL2+''glgC''<br />
 
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'''P6:''' mutagenic PCR to remove EcoRI site 1 from ''glgC''<br />
 
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'''P7:''' mutagenic PCR to remove EcoRI site 2 from ''glgC''<br />
 
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'''P8:''' ''crtB'' (from ''Pantoea ananatis'' cells)<br />
 
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'''P9:''' ''crtI'' (from Douglas's maxiprep of the mutated ''crtIB'' plasmid)<br />
 
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'''P10:''' ''crtY'' (from ''P. ananatis'' cells). Failed.<br />
 
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'''P11:''' fusion PCR for rbs+''dxs''. Failed.<br />
 
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'''P12:''' repeat fusion PCR for rbs+''dxs'', using L11 and primers rbs2fclon1+pSB1A2insr1.<br />
 
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'''P13 and P14:''' MABEL mutation of site 1 on ''glgC'' mutant clones M19 and M22.<br />
 
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'''P15:''' fusion PCR for rbs+''appY''<br />
 
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'''P16, P17, P18 and P19:''' first attempts at ''cenA'', ''cenB'', ''cenC'' and ''cex''.<br />
 
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'''P20, P21, P22 and P23:''' second attempts at ''cenA'', ''cenB'', ''cenC'' and ''cex''.<br />
 
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'''P24, P25:''' repeat PCR for ''cenA'' and ''cex'' using Pfu. Failed.<br />
 
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'''P26:''' Positive control PCR with primers fd1 and rd1 to amplify 16S rRNA gene ''rrnB'' from ''Cellulomonas fimi''.<br />
 
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'''P27:''' ''crtY'' from ''P. ananatis'' cells. - Failed<br />
 
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'''P28, P29:''' ''crtB'' and ''crtI'' from L18 and L19.<br />
 
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'''P30:''' Repeat positive control ''rrnB'' from ''C. fimi'', annealing 1 minute.<br />
 
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'''P31:''' pZntA promoter<br />
 
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'''P32-P35:''' repeat PCR for ''cenA'', ''cenB'', ''cenC'' and ''cex'', annealing 1 minute. Failed.<br />
 
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'''P36:''' M43 (''glgC''-mut1,2) with primers glgCf2/glgCr2 (25.07.08: AM)<br />
 
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'''P37:''' ''crtY'' from ''P. ananatis''. (25.07.08: AM) - Failed<br />
 
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'''P38~P41:''' ''cenA'', ''cenB'', ''cenC'' and ''cex'' from heat-killed cell suspension.<br />
 
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'''P42~P45:''' ''cenA'', ''cenB'', ''cenC'' and ''cex'' from 'impure' DNA solution.<br />
 
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'''P46~P47:''' ''cenA'' and ''cex'' from 'impure' DNA solution, annealing 65C. Failed.<br />
 
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'''P48:''' ''crtY'' (new primer mixture) (previously P38a) (30.07.08: CF) - Failed<br />
 
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'''P49:''' Recreation of P12 (rbs+''dxs'') (previously P39a) (30.07.08: CF)<br />
 
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'''P50:''' third mutagenesis of ''glgc''-mut1,2 (04.08.08: YAN)<br />
 
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'''P51:''' pCstA from ''E. coli'' cells (06.08.08: CF) - Failed<br />
 
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'''P52:''' ''cenA'' from heat killed ''C. fimi'' (07/08/08: YAN, AM) - Failed<br />
 
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'''P53:''' ''cex'' from heat killed ''C. fimi'' (07/08/08: YAN, AM) - Failed<br />
 
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'''P54:''' pCstA from ''E. coli'' cells - Purified (07.08.08: CF)<br />
 

Latest revision as of 13:48, 20 August 2008

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