EPF-Lausanne/21 August 2008

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(Purifying LacIM)
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==Purifying LacIM==
==Purifying LacIM==
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We obtained bad results -> No band visible on the gel. We are then starting again...
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==PCR of Ron Weiss plasmid==
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We have done again a PCR of the Ron Weiss' plasmids with the primers ordered to Invitrogen. We then did a gel electropheresis of the product in order to do a gel purification if the result is good.
==Ligations==
==Ligations==

Revision as of 12:41, 21 August 2008

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Purifying LacIM

We obtained bad results -> No band visible on the gel. We are then starting again...

PCR of Ron Weiss plasmid

We have done again a PCR of the Ron Weiss' plasmids with the primers ordered to Invitrogen. We then did a gel electropheresis of the product in order to do a gel purification if the result is good.

Ligations