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Team:Paris/Modeling/f1
From 2008.igem.org
(Difference between revisions)
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|n<sub>tet</sub> | |n<sub>tet</sub> | ||
| - | |complexation order of pTet<br> | + | |complexation order of pTet<br> n<sub>18</sub> |
|no dimension | |no dimension | ||
| | | | ||
Revision as of 13:55, 21 August 2008
so, with
that gives, after reducing the expression
with the previous expression of [TetR]eq
so, finally, if we use a calculated value of [TetR] (by using the expression of ƒ0 for instance, or by introducing a value given by tetR after an other "caracterised promoter")
| param | signification | unit | value |
| [expr(pTet)] | expression rate of pTet with RBS E0032 | nM.s-1 | see "findparam" need for 20 measures |
| γGFP | dilution-degradation rate of GFP(mut3b) | s-1 | ln(2)/3600 |
| [GFP] | GFP concentration at steady-state | nM | need for 20 measures |
| (fluorescence) | value of the observed fluorescence | au | need for 20 measures |
| conversion | conversion ration between fluorescence and concentration | nM.au-1 | (1/79.429) |
| param | signification corresponding parameters in the equations | unit | value |
| βtet | production rate of pTet with RBS E0032 β1 | nM.s-1 | |
| (Ktet/{coeftetR}ntet) | activation constant of pTet K18 | nMntet | |
| ntet | complexation order of pTet n18 | no dimension | |
| KaTc | complexation constant aTc-TetR K17 | nMnaTc | |
| naTc | complexation order aTc-TetR n17 | no dimension |
