Team:Paris/Modeling/f3
From 2008.igem.org
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Revision as of 14:34, 21 August 2008
At the steady-state, we haveand
so the expression
gives
and
and for calculated values of the TF,
and
param | signification | unit | value |
[expr(pFlhDC)] | expression rate of pFlhDC with RBS E0032 | nM.s-1 | see "findparam" need for 20 + 20 measures and 5x5 measures for the SUM? |
γGFP | dilution-degradation rate of GFP(mut3b) | s-1 | ln(2)/3600 |
[GFP] | GFP concentration at steady-state | nM | need for 20 measures |
(fluorescence) | value of the observed fluorescence | au | need for 20 + 20 measures and 5x5 measures for the SUM? |
conversion | conversion ration between fluorescence and concentration | nM.au-1 | (1/79.429) |
param | signification corresponding parameters in the equations | unit | value |
βbad | production rate of pFlhDC with RBS E0032 not in the system | nM.s-1 | |
(Kbad/[AraCtot]nbad) | activation constant of pBad not in the system | nMnbad | |
nbad | complexation order of pBad not in the system | no dimension | |
Kara | complexation constant Arabinose-AraC not in the system | nMnara | |
nara | complexation order Arabinose-AraC n17 | no dimension |