Team:Paris/Modeling/f3

From 2008.igem.org

(Difference between revisions)
Line 42: Line 42:
|GFP concentration at steady-state
|GFP concentration at steady-state
|nM
|nM
-
|need for 20 measures
+
|need for 20 + 20 measures <br> and 5x5 measures for the ''SUM''?
|-
|-
|(''fluorescence'')
|(''fluorescence'')
Line 63: Line 63:
|value
|value
|-
|-
-
|β<sub>bad</sub>
+
|β<sub>12</sub>
-
|production rate of pFlhDC '''with RBS E0032''' <br> not in the system
+
|production rate of FliA-pFlhDC '''with RBS E0032''' <br> β<sub>12</sub>
|nM.s<sup>-1</sup>
|nM.s<sup>-1</sup>
|
|
|-
|-
-
|(K<sub>bad</sub>/[AraC<sub>tot</sub>]<sup>n<sub>bad</sub></sup>)
+
|(K<sub>11</sub>/{coef<sub>fliA</sub>}<sup>n<sub>11</sub></sup>)
-
|activation constant of pBad <br> not in the system
+
|activation constant of FliA-pFlhDC <br> K<sub>11</sub>
-
|nM<sup>n<sub>bad</sub></sup>
+
|nM<sup>n<sub>11</sub></sup>
|
|
|-
|-
-
|n<sub>bad</sub>
+
|n<sub>11</sub>
-
|complexation order of pBad<br> not in the system
+
|complexation order of FliA-pFlhDC <br> n<sub>11</sub>
|no dimension
|no dimension
|
|
|-
|-
-
|K<sub>ara</sub>
+
|-
-
|complexation constant Arabinose-AraC <br> not in the system
+
|β<sub>2</sub>
-
|nM<sup>n<sub>ara</sub></sup>
+
|production rate of OmpR-pFlhDC '''with RBS E0032''' <br> β<sub>2</sub>
 +
|nM.s<sup>-1</sup>
 +
|
 +
|-
 +
|(K<sub>11</sub>/{coef<sub>ompR</sub>}<sup>n<sub>19</sub></sup>)
 +
|activation constant of OmpR-pFlhDC <br> K<sub>19</sub>
 +
|nM<sup>n<sub>19</sub></sup>
|
|
|-
|-
-
|n<sub>ara</sub>
+
|n<sub>19</sub>
-
|complexation order Arabinose-AraC <br> n<sub>17</sub>
+
|complexation order of OmpR-pFlhDC <br> n<sub>19</sub>
|no dimension
|no dimension
|
|
|}
|}

Revision as of 14:45, 21 August 2008

F3omp.jpg
At the steady-state, we have
FliAeq.jpg

and

OmpReq.jpg

so the expression

F3expr.jpg

gives

F3FliA.jpg

and

F3OmpR.jpg

and for calculated values of the TF,

F3FliACalc.jpg

and

F3OmpRCalc.jpg



param signification unit value
[expr(pFlhDC)] expression rate of
pFlhDC with RBS E0032 		nM.s-1 see "findparam"
need for 20 + 20 measures
and 5x5 measures for the SUM?
γGFP dilution-degradation rate
of GFP(mut3b) 		s-1 ln(2)/3600
[GFP] GFP concentration at steady-state nM need for 20 + 20 measures
and 5x5 measures for the SUM?
(fluorescence) value of the observed fluorescence au need for 20 + 20 measures
and 5x5 measures for the SUM?
conversion conversion ration between
fluorescence and concentration 		nM.au-1 (1/79.429)



param signification
corresponding parameters in the equations 		unit value
β12 production rate of FliA-pFlhDC with RBS E0032
β12 		nM.s-1
(K11/{coeffliA}n11) activation constant of FliA-pFlhDC
K11 		nMn11
n11 complexation order of FliA-pFlhDC
n11 		no dimension
β2 production rate of OmpR-pFlhDC with RBS E0032
β2 		nM.s-1
(K11/{coefompR}n19) activation constant of OmpR-pFlhDC
K19 		nMn19
n19 complexation order of OmpR-pFlhDC
n19 		no dimension
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