Team:Paris/Modeling/f6

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(Difference between revisions)

Revision as of 16:25, 21 August 2008

At the steady-state, we have

and

so the expression

gives

and

and for calculated values of the TF,

and

param	signification	unit	value
[expr(pFlgA)]	expression rate of pFlgA with RBS E0032	nM.s^-1	see "findparam" need for 20 + 20 measures and 5x5 measures for the SUM?
γ_GFP	dilution-degradation rate of GFP(mut3b)	s^-1	ln(2)/3600
[GFP]	GFP concentration at steady-state	nM	need for 20 + 20 measures and 5x5 measures for the SUM?
(fluorescence)	value of the observed fluorescence	au	need for 20 + 20 measures and 5x5 measures for the SUM?
conversion	conversion ration between fluorescence and concentration	nM.au^-1	(1/79.429)

param	signification corresponding parameters in the equations	unit	value
β₅₅	production rate of FlhDC-pFlgA with RBS E0032 β₅₅	nM.s^-1
(K₄₉/{coef_flhDC}^n₄₉)	activation constant of FlhDC-pFlgA K₄₉	nM^n₄₉
n₄₉	complexation order of FliA-pFlgA n₄₉	no dimension
β₅₆	production rate of FliA-pFlgA with RBS E0032 β₅₆	nM.s^-1
(K₅₀/{coef_fliA}^n₅₀)	activation constant of FliA-pFlgA K₅₀	nM^n₅₀
n₅₀	complexation order of FliA-pFlgA n₅₀	no dimension

Then, if we have time, we want to verify the expected relation

@@ Line 29: / Line 29: @@
 |value
 |-
-|[expr(pFliL)]
+|[expr(pFlgA)]
-|expression rate of <br> pFliA '''with RBS E0032'''
+|expression rate of <br> pFlgA '''with RBS E0032'''
 |nM.s<sup>-1</sup>
 |see [[Team:Paris/Modeling/Programs|"findparam"]] <br> need for 20 + 20 measures <br> and 5x5 measures for the ''SUM''?
@@ Line 63: / Line 63: @@
 |value
 |-
-|β<sub>53</sub>
+|β<sub>55</sub>
-|production rate of FlhDC-pFliL '''with RBS E0032''' <br> β<sub>53</sub>
+|production rate of FlhDC-pFlgA '''with RBS E0032''' <br> β<sub>55</sub>
 |nM.s<sup>-1</sup>
 |
 |-
-|(K<sub>47</sub>/{coef<sub>flhDC</sub>}<sup>n<sub>47</sub></sup>)
+|(K<sub>49</sub>/{coef<sub>flhDC</sub>}<sup>n<sub>49</sub></sup>)
-|activation constant of FlhDC-pFliL <br> K<sub>47</sub>
+|activation constant of FlhDC-pFlgA <br> K<sub>49</sub>
-|nM<sup>n<sub>47</sub></sup>
+|nM<sup>n<sub>49</sub></sup>
 |
 |-
-|n<sub>47</sub>
+|n<sub>49</sub>
-|complexation order of FliA-pFliL <br> n<sub>47</sub>
+|complexation order of FliA-pFlgA <br> n<sub>49</sub>
 |no dimension
 |
 |-
 |-
-|β<sub>54</sub>
+|β<sub>56</sub>
-|production rate of FliA-pFliL '''with RBS E0032''' <br> β<sub>54</sub>
+|production rate of FliA-pFlgA '''with RBS E0032''' <br> β<sub>56</sub>
 |nM.s<sup>-1</sup>
 |
 |-
-|(K<sub>48</sub>/{coef<sub>fliA</sub>}<sup>n<sub>48</sub></sup>)
+|(K<sub>50</sub>/{coef<sub>fliA</sub>}<sup>n<sub>50</sub></sup>)
-|activation constant of OmpR-pFlhDC <br> K<sub>48</sub>
+|activation constant of FliA-pFlgA <br> K<sub>50</sub>
-|nM<sup>n<sub>48</sub></sup>
+|nM<sup>n<sub>50</sub></sup>
 |
 |-
-|n<sub>48</sub>
+|n<sub>50</sub>
-|complexation order of OmpR-pFlhDC <br> n<sub>48</sub>
+|complexation order of FliA-pFlgA <br> n<sub>50</sub>
 |no dimension
 |