EPF-Lausanne/21 August 2008

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(Purifying LacIM)
 
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==PCR of Ron Weiss plasmid==
==PCR of Ron Weiss plasmid==
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We have done again a PCR of the Ron Weiss' plasmids with the primers ordered to Invitrogen. We then did a gel electropheresis of the product in order to do a gel purification if the result is good.
+
We have done again a PCR of the Ron Weiss' plasmids with the primers ordered to Invitrogen. We then did a gel electropheresis of the product, which reveals that the PCR had not worked.
 +
We therefore started the PCR again, with still 9 aliquots (each plasmid PHD1,2,3 with 3 different concentrations of primers) with exactly the same protocol as the one used when ti works. Hopefully, it will work this time... See tomorrow with a gel.
==Ligations==
==Ligations==
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 +
The ligations worked!
 +
 +
We have now:
 +
 +
===GL===
 +
I14033 + I1466
 +
 +
===VL===
 +
C0040 + B0015

Latest revision as of 12:24, 26 August 2008

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Contents

Purifying LacIM

We obtained bad results -> No band visible on the gel. We are then starting again...

PCR of Ron Weiss plasmid

We have done again a PCR of the Ron Weiss' plasmids with the primers ordered to Invitrogen. We then did a gel electropheresis of the product, which reveals that the PCR had not worked. We therefore started the PCR again, with still 9 aliquots (each plasmid PHD1,2,3 with 3 different concentrations of primers) with exactly the same protocol as the one used when ti works. Hopefully, it will work this time... See tomorrow with a gel.

Ligations

The ligations worked!

We have now:

GL

I14033 + I1466

VL

C0040 + B0015